利用聚合酶链反应检测阿米巴肝脓肿脓液样本中的溶组织内阿米巴。
Detection of Entamoeba histolytica using polymerase chain reaction in pus samples from amebic liver abscess.
作者信息
Khan Ujjawini, Mirdha B R, Samantaray J C, Sharma M P
机构信息
Department of Microbiology, All India Institute of Medical Sciences, Ansari Nagar, New Delhi , India.
出版信息
Indian J Gastroenterol. 2006 Mar-Apr;25(2):55-7.
BACKGROUND AND OBJECTIVE
Direct demonstration of Entamoeba histolytica by conventional microscopy and in vitro culture in pus obtained from amebic liver abscess (ALA) is often unsuccessful. We evaluated polymerase chain reaction (PCR) for detection of E. histolytica DNA in such pus.
METHODS
Species-specific primers were used for the amplification of E. histolytica DNA from liver pus obtained from 30 patients with ALA. Patients with pyogenic liver abscess and sterile (autoclaved) pus spiked with Entamoeba dispar and bacteria (Escherichia coli, Klebsiella spp. and Bacteroides spp.) were used as negative controls.
RESULTS
PCR was positive in 83% of pus specimens from patients with ALA, and was negative in all 25 pus specimens obtained from pyogenic abscess and autoclaved pus spiked with known bacteria. Sensitivity and specificity of PCR were 83% and 100%, respectively. The overall positivity of PCR was higher compared to serological tests.
CONCLUSION
PCR may be a more reliable and better alternative diagnostic modality for ALA.
背景与目的
通过传统显微镜检查以及对从阿米巴肝脓肿（ALA）获取的脓液进行体外培养来直接检测溶组织内阿米巴，常常难以成功。我们评估了聚合酶链反应（PCR）用于检测此类脓液中溶组织内阿米巴DNA的情况。
方法
使用种特异性引物，从30例ALA患者的肝脓液中扩增溶组织内阿米巴DNA。将患有化脓性肝脓肿的患者以及接种了溶组织内阿米巴和细菌（大肠杆菌、克雷伯菌属和拟杆菌属）的无菌（高压灭菌）脓液用作阴性对照。
结果
PCR在83%的ALA患者脓液标本中呈阳性，而在从化脓性脓肿获取的所有25份脓液标本以及接种了已知细菌的高压灭菌脓液中均呈阴性。PCR的敏感性和特异性分别为83%和100%。与血清学检测相比，PCR的总体阳性率更高。
结论
对于ALA，PCR可能是一种更可靠且更好的替代诊断方法。
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