Schawalder Alissa, Hue-Roye Kim, Castilho Lilian, Chaudhuri Asok, Reid Marion E
Immunohematology and Immunochemisry Laboratories, New York Blood Center, New York, NY 10021, USA.
J Med Primatol. 2006 Jun;35(3):144-8. doi: 10.1111/j.1600-0684.2006.00160.x.
The anion exchanger, Band 3, carries antigens in the Diego blood group system, and can carry the Band 3-Memphis phenotype. Although Di(b) is of high prevalence and Band 3-Memphis is of low prevalence in humans, it has been suggested that both are on the ancestral gene. We determined the orthologue nucleotide sequences corresponding to these two polymorphic sites, Di(a)/Di(b) (2561T > C; Leu854Pro) and Band 3-Memphis(166A > G; Lys56Glu) in several nonhuman primates.
Genomic DNA was extracted from blood samples of great apes, lesser apes, old world monkeys, new world monkeys and prosimians. PCR amplifications were done with primer pairs that were located in the flanking intronic regions of Exon 4 and Exon 19; and the amplified products were sequenced.
Amino acid sequence alignment of nonhuman primates band 3 with that of human showed extensive homologies. In exon 4, Glu56Lys polymorphic site showed Glu similar to Band 3-Memphis type and in exon 19, Leu854Pro polymorphic site showed Pro indicating Di(b) phenotype.
The nonhuman primates have nucleotide sequences of Di(b)(2561C) in cis to Band 3-Memphis (166G), which is consistent with the assertion that the Di(b) and Band 3-Memphis phenotype represents the ancestral Band 3 gene.
阴离子交换蛋白3（Band 3）携带迭戈血型系统中的抗原，并可呈现Band 3 - 孟菲斯表型。尽管Di(b)在人类中具有高流行率，而Band 3 - 孟菲斯具有低流行率，但有人提出两者都存在于祖先基因上。我们确定了几种非人类灵长类动物中与这两个多态性位点相对应的直系同源核苷酸序列，即Di(a)/Di(b)（2561T > C；Leu854Pro）和Band 3 - 孟菲斯（166A > G；Lys56Glu）。
从大猩猩、长臂猿、旧世界猴、新世界猴和原猴亚目的血液样本中提取基因组DNA。使用位于外显子4和外显子19侧翼内含子区域的引物对进行PCR扩增；并对扩增产物进行测序。
非人类灵长类动物Band 3与人类的氨基酸序列比对显示出广泛的同源性。在外显子4中，Glu56Lys多态性位点显示为Glu，类似于Band 3 - 孟菲斯类型；在外显子19中，Leu854Pro多态性位点显示为Pro，表明为Di(b)表型。
非人类灵长类动物具有与Band 3 - 孟菲斯（166G）顺式排列的Di(b)（2561C）核苷酸序列，这与Di(b)和Band 3 - 孟菲斯表型代表祖先Band 3基因的说法一致。
