Yegin Betül Arica, Lamprecht Alf
Department of Pharmaceutical Technology, Faculty of Pharmacy, Hacettepe University, 06100 Sihhiye-Ankara, Turkey.
Int J Pharm. 2006 Aug 31;320(1-2):165-70. doi: 10.1016/j.ijpharm.2006.04.014. Epub 2006 Apr 30.
Particle size and the size distribution are known to be important factors in the overall behavior of nanoparticulate drug delivery systems and an exact determination of these parameters is highly important. Several techniques are applied in the size determination of nanoparticulates, particularly dynamic light scattering. Also other methods have been proposed, among them hydrodynamic chromatography (HDC). In order to characterize a HDC method for nanosized carriers, differently sized lipid nanocapsules having a diameter of 25-100 nm were analyzed and results were compared with measurements from photon correlation spectroscopy (PCS) and atomic force microscopy. Results from atomic force microscopy studies were generally not in line with determinations from the other techniques due to a particle shape loss by the drying step. PCS and HDC led to comparable results in simple size determination of lipid nanocapsules. However, slight differences were found during the characterization of more complex samples. HDC was able to detect micelles as a byproduct of nanocapsule preparation while in PCS the sample dilution turned micelles undetectable. HDC analysis was able to characterize mixed samples of particle batches differing in their average size similar to PCS. HDC was found to be an excellent tool for the determination of size and average size distribution of lipid nanocapsules.
众所周知，粒径和粒径分布是纳米颗粒药物递送系统整体行为的重要因素，准确测定这些参数至关重要。有几种技术可用于测定纳米颗粒的大小，特别是动态光散射。还提出了其他方法，其中包括流体动力学色谱法（HDC）。为了表征一种用于纳米载体的HDC方法，分析了直径为25-100nm的不同大小的脂质纳米胶囊，并将结果与光子相关光谱法（PCS）和原子力显微镜的测量结果进行了比较。由于干燥步骤导致颗粒形状损失，原子力显微镜研究的结果通常与其他技术的测定结果不一致。在脂质纳米胶囊的简单大小测定中，PCS和HDC得出了可比的结果。然而，在表征更复杂的样品时发现了细微差异。HDC能够检测到作为纳米胶囊制备副产物的胶束，而在PCS中，样品稀释使胶束无法检测到。与PCS类似，HDC分析能够表征平均大小不同的颗粒批次的混合样品。结果发现，HDC是测定脂质纳米胶囊大小和平均大小分布的极佳工具。
