Rudek Michelle A, Zhao Ming, He Ping, Messersmith Wells A, Baker Sharyn D
The Sidney Kimmel Comprehensive Cancer Center at Johns Hopkins, Baltimore, MD, USA.
J Pharm Biomed Anal. 2006 Sep 18;42(2):253-60. doi: 10.1016/j.jpba.2006.04.010. Epub 2006 Jun 9.
A method has been developed for the quantitation of ABT-751, ABT-751 glucuronide, and ABT-751 sulfate in human plasma. ABT-751 and metabolites were separated from endogenous material on a C18 column with acetonitrile-ammonium acetate (2 mM) mobile phase containing formic acid (0.1%, v/v) using isocratic flow for 5 min. The analytes were monitored by tandem-mass spectrometry. Calibration curves were generated over the range of 20-5,000 ng/ml for ABT-751, ABT-751 glucuronide, and ABT-751 sulfate. A 20,000 ng/ml sample that was diluted 1:10 (v/v) with plasma was accurately quantitated. The method has been successfully applied to study the plasma pharmacokinetics of ABT-751 in humans.
已开发出一种用于定量测定人血浆中ABT - 751、ABT - 751葡萄糖醛酸苷和ABT - 751硫酸盐的方法。使用含甲酸（0.1%，v/v）的乙腈 - 乙酸铵（2 mM）流动相，在C18柱上通过等度洗脱5分钟，将ABT - 751及其代谢物与内源性物质分离。通过串联质谱法监测分析物。针对ABT - 751、ABT - 751葡萄糖醛酸苷和ABT - 751硫酸盐，在20 - 5000 ng/ml范围内生成校准曲线。对用血浆按1:10（v/v）稀释的20000 ng/ml样品进行了准确定量。该方法已成功应用于研究ABT - 751在人体中的血浆药代动力学。
