Gao Shouhong, Tao Xia, Sun Lianna, Sheng Chunquan, Zhang Wannian, Yun Yunlei, Li Jingxian, Miao Haijun, Chen Wansheng
Department of Pharmacy, Changzheng Hospital, Second Military Medical University, No. 415 Fengyang Road, Shanghai 200003, PR China.
J Chromatogr B Analyt Technol Biomed Life Sci. 2009 Feb 1;877(4):382-6. doi: 10.1016/j.jchromb.2008.12.034. Epub 2008 Dec 24.
A simple, rapid and sensitive LC-MS/MS method for determination of trace amount of new antifungal drug iodiconazole in human plasma was developed (1-(1H-1,2,4-triazole)-2-(2,4-diflurophenyl)-3-[N-methyl-N-(3-chlor-benzyl)amino]-2-propanol), was used as internal standard (IS). The analytes were extracted by liquid-liquid extraction with n-hexane after internal standard spiked. The separation was performed by a ZORBAX SB-C(18) column (3.5 microm, 2.1 mm x 100 mm) with an isocratic mobile phase consisting of methanol-water-formic acid (50:50:0.05, v/v/v) at a flow rate of 0.3 mL/min. The lower limit of quantification (LLOQ) was 0.10 ng/mL. This method was successfully used to determine the concentration of iodiconazole in human plasma following dermal administration.
建立了一种简单、快速且灵敏的液相色谱-串联质谱法，用于测定人血浆中痕量新型抗真菌药物碘康唑（1-(1H-1,2,4-三唑)-2-(2,4-二氟苯基)-3-[N-甲基-N-(3-氯苄基)氨基]-2-丙醇），使用其作为内标（IS）。内标加入后，通过正己烷液-液萃取法提取分析物。采用ZORBAX SB-C(18)柱（3.5微米，2.1毫米×100毫米）进行分离，等度流动相由甲醇-水-甲酸（50:50:0.05，v/v/v）组成，流速为0.3毫升/分钟。定量下限（LLOQ）为0.10纳克/毫升。该方法成功用于测定经皮给药后人血浆中碘康唑的浓度。
