Gómez Eva, Schulman Alan H, Martínez-Izquierdo José Antonio, Vicient Carlos M
Department of Molecular Genetics, Consorci Consejo Superior de Investigaciones Cientificas - Institut de Recerca i Tecnologia Agroalimentàries, Jordi Girona, Barcelona, Spain.
Genome. 2006 May;49(5):558-62. doi: 10.1139/g05-129.
Grande is an abundant gypsy-like retrotransposon present in the genera Zea and Tripsacum. Related retro transposon families can be found in sorghum, rice, and barley, but not in wheat or rye. We have amplified and sequenced several copies of part of the integrase domain derived from the Zea mays, Zea diploperennis, and Tripsacum dactyloides genomes. There are no significant differences in divergence or clustering between the integrase sequences of these species. The substitution rate for synonimous sites was found to be higher than those of non-synomymous sites; this indicates that Grande integrase has been under purifying selection for function. Grande is transcribed in leaves. The transcripts show sequence diversity similar to that of genomic sequences, but belong to restricted clades; this indicates that only some evolutionary branches of Grande have retained transcriptional competence.
格兰德（Grande）是一种存在于玉米属和摩擦禾属中的丰富的类吉普赛逆转录转座子。在高粱、水稻和大麦中可以发现相关的逆转录转座子家族，但在小麦或黑麦中则没有。我们从玉米、二倍体多年生玉米和大刍草的基因组中扩增并测序了整合酶结构域部分的几个拷贝。这些物种的整合酶序列在分歧或聚类方面没有显著差异。同义位点的替换率高于非同义位点；这表明格兰德整合酶在功能上一直受到纯化选择。格兰德在叶片中表达。转录本显示出与基因组序列相似的序列多样性，但属于有限的进化枝；这表明格兰德只有一些进化分支保留了转录能力。
