Matsubara Hisashi, Kuze Manami, Sasoh Mikio, Ma Ning, Furuta Motoyasu, Uji Yukitaka
Department of Ophthalmology, Mie University School of Medicine, Tsu, Mie, Japan.
Jpn J Ophthalmol. 2006 May-Jun;50(3):211-6. doi: 10.1007/s10384-005-0315-8.
This study evaluates the relevance to human retinopathy of electroretinograms (ERGs) from the spontaneously diabetic Goto-Kakizaki (GK) rat.
Starting from 4 weeks of age, we obtained ERGs every 4 weeks from six GK rats and seven Wistar (control) rats, and from two GK and two Wistar rats at 14 days of age. The a-wave, b-wave, and oscillatory potentials (OPs) were recorded after stimulation with a single bright flash. We compared the amplitudes and implicit times and measured a-wave latencies to evaluate photoreceptor function.
The amplitudes of the a-wave, b-wave, and OPs (O1 and O2) of the GK rats were reduced between 4 and 48 weeks of age. The time-dependent courses of change in a-wave, b-wave, and O2 amplitude did not differ between the two groups. The a-wave latencies in GK rats were significantly prolonged, but not the implicit times of OPs. At 14 days of age, the a-wave amplitudes were significantly smaller in GK than in Wistar rats.
Functional abnormalities of photoreceptors might be induced by inheritable degeneration at an early age in the GK rat. Although hyperglycemia would cause retinal hypoxia, it would not be severe enough to disturb the generation of OPs.
本研究评估自发性糖尿病Goto-Kakizaki（GK）大鼠的视网膜电图（ERG）与人类视网膜病变的相关性。
从4周龄开始，我们每4周从6只GK大鼠和7只Wistar（对照）大鼠身上获取ERG，并在14日龄时从2只GK大鼠和2只Wistar大鼠身上获取。用单次明亮闪光刺激后记录a波、b波和振荡电位（OPs）。我们比较了振幅和隐含时间，并测量了a波潜伏期以评估光感受器功能。
GK大鼠的a波、b波和OPs（O1和O2）振幅在4至48周龄之间降低。两组之间a波、b波和O2振幅随时间的变化过程没有差异。GK大鼠的a波潜伏期显著延长，但OPs的隐含时间没有延长。在14日龄时，GK大鼠的a波振幅明显小于Wistar大鼠。
GK大鼠早期可能因遗传性变性而导致光感受器功能异常。虽然高血糖会导致视网膜缺氧，但程度不足以干扰OPs的产生。
