谷氨酸诱导培养星形胶质细胞肿胀的形态学研究：钙和氯离子机制的参与

A morphological study on glutamate-induced swelling of cultured astrocytes: involvement of calcium and chloride ion mechanisms.

作者信息

Koyama Y, Sugimoto T, Shigenaga Y, Baba A, Iwata H

机构信息

Department of Pharmacology, Faculty of Pharmaceutical Sciences, Osaka University, Japan.

出版信息

Neurosci Lett. 1991 Apr 1;124(2):235-8. doi: 10.1016/0304-3940(91)90102-y.

DOI:10.1016/0304-3940(91)90102-y

PMID:1676835

Abstract

Morphological changes in cultured astrocytes exposed to L-glutamate (Glu) were examined light and electron microscopically. The treatment with 0.1 mM Glu for 60 min caused marked swelling of the cells, which was characterized by reduction in staining of cytoplasm with Toluidine blue, disappearance of the cytoplasmic granular ground substances, swollen mitochondrion and nucleus, and dispersed chromatin. The above changes were prevented by the removal of Na+, Ca2+ or Cl- from the incubation medium for Glu treatment. However, the Glu treatment in a Cl(-)-free medium caused conspicuous aggregation of 10 nm filaments.

摘要

用光镜和电镜检查了暴露于L-谷氨酸（Glu）的培养星形胶质细胞的形态学变化。用0.1 mM Glu处理60分钟导致细胞明显肿胀，其特征为甲苯胺蓝对细胞质的染色减少、细胞质颗粒状基质消失、线粒体和细胞核肿胀以及染色质分散。通过从用于Glu处理的孵育培养基中去除Na+、Ca2+或Cl-可防止上述变化。然而，在无Cl-的培养基中进行Glu处理会导致10 nm细丝明显聚集。