Arinaga K, Rant U, Tornow M, Fujita S, Abstreiter G, Yokoyama N
Walter Schottky Institut, Technische Universitaet Muenchen, 85748 Garching, Germany.
Langmuir. 2006 Jun 20;22(13):5560-2. doi: 10.1021/la060324m.
We study the coadsorption of mercaptohexanol onto preimmobilized oligonucleotide layers on gold. Monitoring the position of the DNA relative to the surface by optical means directly shows the mercaptohexanol-induced desorption of DNA and the reorientation of surface-tethered strands in situ and in real time. By simultaneously recording the electrochemical electrode potential, we are able to demonstrate that changes in the layer conformation are predominantly of electrostatic origin and can be reversed by applying external bias to the substrate.
我们研究了巯基己醇在预先固定于金表面的寡核苷酸层上的共吸附情况。通过光学手段监测DNA相对于表面的位置，直接显示了巯基己醇诱导的DNA解吸以及表面连接链原位实时重新取向。通过同时记录电化学电极电位，我们能够证明层构象的变化主要源于静电作用，并且可以通过向基底施加外部偏压来逆转。
