一种使用Mupid-2迷你凝胶电泳装置改进的Southern DNA印迹和Northern RNA印迹方法。

An improved method for Southern DNA and Northern RNA blotting using a Mupid-2 Mini-Gel electrophoresis unit.

作者信息

Furuya Hirokazu, Yamada Takeshi, Ikezoe Koji, Ohyagi Yasumasa, Fukumaki Yasuyuki, Fujii Naoki

机构信息

Clinical Research Center, Japan.

出版信息

J Biochem Biophys Methods. 2006 Aug 31;68(2):139-43. doi: 10.1016/j.jbbm.2006.04.005.

DOI:10.1016/j.jbbm.2006.04.005

PMID:16769124

Abstract

An improved method for Southern DNA and Northern RNA blotting using the Mupid-2 Mini-Gel System is described. We get sharp and clear bands in Southern and Northern blotting after only 30 min short gel electrophoresis instead of the several hours large gel electrophoresis of conventional methods. The high electrical voltage with a pulse-like current of the Mupid-2 Mini-Gel System also allows reduction of the amount of formaldehyde, a harmful reagent, from the gel running buffer in RNA blotting. This minor modification of DNA and RNA blotting technique enables us to perform the complete experimental procedure more quickly economically in less space, than conventional Southern and Northern blotting, as well as using an extremely small amount of formaldehyde in RNA blotting.

摘要

本文描述了一种使用Mupid-2迷你凝胶系统改进的Southern DNA印迹法和Northern RNA印迹法。仅通过30分钟的短凝胶电泳，我们就在Southern和Northern印迹中获得了清晰锐利的条带，而不是传统方法中需要数小时的大凝胶电泳。Mupid-2迷你凝胶系统的高电压和脉冲状电流还使得RNA印迹中凝胶运行缓冲液中有害试剂甲醛的用量得以减少。DNA和RNA印迹技术的这一微小改进使我们能够比传统的Southern和Northern印迹法在更小的空间内更快速、经济地完成整个实验过程，同时在RNA印迹中使用极少量的甲醛。