McDonnell Liam A, Heeren Ron M A, de Lange Robert P J, Fletcher Ian W
FOM Institute for Atomic and Molecular Physics, Amsterdam, The Netherlands.
J Am Soc Mass Spectrom. 2006 Sep;17(9):1195-202. doi: 10.1016/j.jasms.2006.05.003. Epub 2006 Jun 12.
To expand the role of high spatial resolution secondary ion mass spectrometry (SIMS) in biological studies, numerous developments have been reported in recent years for enhancing the molecular ion yield of high mass molecules. These include both surface modification, including matrix-enhanced SIMS and metal-assisted SIMS, and polyatomic primary ions. Using rat brain tissue sections and a bismuth primary ion gun able to produce atomic and polyatomic primary ions, we report here how the sensitivity enhancements provided by these developments are additive. Combined surface modification and polyatomic primary ions provided approximately 15.8 times more signal than using atomic primary ions on the raw sample, whereas surface modification and polyatomic primary ions yield approximately 3.8 and approximately 8.4 times more signal. This higher sensitivity is used to generate chemically specific images of higher mass biomolecules using a single molecular ion peak.
为了扩大高空间分辨率二次离子质谱（SIMS）在生物学研究中的作用，近年来已有许多关于提高高质量分子分子离子产率的进展报道。这些进展包括表面改性，如基质增强SIMS和金属辅助SIMS，以及多原子一次离子。使用大鼠脑组织切片和能够产生原子和多原子一次离子的铋一次离子枪，我们在此报告这些进展所提供的灵敏度增强是可叠加的。与在原始样品上使用原子一次离子相比，表面改性和多原子一次离子相结合产生的信号大约多15.8倍，而表面改性和多原子一次离子分别产生的信号大约多3.8倍和大约8.4倍。这种更高的灵敏度被用于利用单个分子离子峰生成高质量生物分子的化学特异性图像。
