Qi Wang, Yongjun Jiang, Yanan Wang, Zining Zhang, Xiaoxu Han, Jing Liu, Hong Shang
Shenyang Center for Disease Control and Prevention, Shenyang, Liaoning, People's Republic of China.
J Clin Immunol. 2006 Jul;26(4):339-46. doi: 10.1007/s10875-006-9027-1. Epub 2006 Jun 13.
Cytotoxic lymphocytes are critical in the control of HIV replication, it has been shown that perforin is the key effector of killing machinery for CTLs and NK cells, so we investigated the circulating levels of perforin in CD8+ T cells and NK cells by flow cytometry intracellular stain in Chinese HIV infected individuals, its association with disease progression was analyzed. Our results showed that NK cells express perforin more efficiently than CD8+ T cells, CD8+ T cells expressed perforin higher than that of healthy controls, but NK cells expressed lower perforin than that of healthy controls, both were not correlated with disease progression. but significantly associated with their numbers, anti-retrovirus therapy had no evident effects on peforin expression in CD8+ T cells, but enhanced perfrin expression in NK cells, perforin expression in CD8+ T cells and CD16+ NK cells correlate with CD4+ T cell counts significantly in HAART-treated group. Therefore, different mechanisms may be involved in regulating peripheral perforin expression in different cell types.
细胞毒性淋巴细胞在控制HIV复制中起关键作用，研究表明穿孔素是细胞毒性T淋巴细胞（CTL）和自然杀伤细胞（NK细胞）杀伤机制的关键效应分子，因此我们通过流式细胞术细胞内染色检测了中国HIV感染者CD8+ T细胞和NK细胞中穿孔素的循环水平，并分析了其与疾病进展的关系。我们的结果显示，NK细胞比CD8+ T细胞更有效地表达穿孔素，CD8+ T细胞的穿孔素表达高于健康对照，但NK细胞的穿孔素表达低于健康对照，两者均与疾病进展无关，但与它们的数量显著相关，抗逆转录病毒疗法对CD8+ T细胞中穿孔素的表达没有明显影响，但增强了NK细胞中穿孔素的表达，在接受高效抗逆转录病毒治疗（HAART）的组中，CD8+ T细胞和CD16+ NK细胞中的穿孔素表达与CD4+ T细胞计数显著相关。因此，不同的机制可能参与调节不同细胞类型中外周穿孔素的表达。
