Kanzaki Masato, Yamato Masayuki, Hatakeyama Hideyuki, Kohno Chinatsu, Yang Joseph, Umemoto Terumasa, Kikuchi Akihiko, Okano Teruo, Onuki Takamasa
The Department of Surgery I, Tokyo Women's Medical University, Tokyo, Japan.
Tissue Eng. 2006 May;12(5):1275-83. doi: 10.1089/ten.2006.12.1275.
To successfully engineer a bioartificial tracheal replacement, it is believed that the regeneration of a functional epithelial lining is a key requirement. In the present study, rabbit tracheal epithelial cells were cultured on temperature-responsive culture dishes, under normal culture conditions at 37 degrees C. By simple temperature reduction to 20 degrees C, the cultured epithelial cells were noninvasively harvested as intact sheets, without the use of any proteolytic enzymes. Support Dacron grafts that had been subcutaneously implanted for 4 weeks to allow for host tissue and vessel infiltration were then opened, and the tracheal epithelial cell sheets were transplanted to the luminal surface without sutures. These fabricated constructs were then used as tracheal replacements, in a rabbit model. Four weeks after transplantation, results showed that the tracheal grafts were covered by a mature, pseudostratified columnar epithelium. In contrast, control constructs that did not receive cell sheet transplantation demonstrated only a thin, immature epithelium at the center of the replacement graft. These results therefore demonstrate that these tracheal epithelial cell sheets can create an epithelial lining on the luminal surface of a bioartificial trachea.
为成功构建生物人工气管替代物，人们认为功能性上皮衬里的再生是一项关键要求。在本研究中，兔气管上皮细胞在温度响应培养皿中于37℃的正常培养条件下培养。通过简单地将温度降至20℃，无需使用任何蛋白水解酶，即可无创收获完整的培养上皮细胞片。然后打开已皮下植入4周以允许宿主组织和血管浸润的支撑涤纶移植物，将气管上皮细胞片无缝合地移植到管腔表面。然后将这些构建物用作兔模型中的气管替代物。移植4周后，结果显示气管移植物被成熟的假复层柱状上皮覆盖。相比之下，未接受细胞片移植的对照构建物在替代移植物中心仅显示出薄的、不成熟的上皮。因此，这些结果表明，这些气管上皮细胞片可在生物人工气管的管腔表面形成上皮衬里。
