Department of Otolaryngology, School of Medicine, Fukushima Medical University, 1 Hikarigaoka, Fukushima City 960-1295, Japan.
Biomaterials. 2010 Jun;31(18):4855-63. doi: 10.1016/j.biomaterials.2010.02.027. Epub 2010 Mar 26.
In some types of tracheal disease, tracheal resection is required. For patients with tracheal resection, artificial grafts, made from collagen sponge with a spiral polypropylene stent and mesh, have been clinically used by our group. However, epithelial regeneration was confirmed to be slow. In the present study, we investigated the potential of gingival fibroblasts (GFBs) and adipose-derived stem cells (ASCs) as autologous transplanted cells in combination with artificial graft for tracheal epithelial regeneration. In in vitro co-culturing with tracheal epithelial cells, GFBs stimulated epithelial cell differentiation and reconstruction of a pseudostratified epithelium. ASCs stimulated epithelial cell proliferation and reconstruction of a multi-layered epithelium. Subsequently, we prepared three kinds of bioengineered scaffolds from GFBs and/or ASCs and implanted them into rat tracheal defects. The bioengineered scaffolds containing GFBs were covered with tracheal epithelial cells after 1 week, and highly ciliated epithelium was formed after 2 weeks of transplantation. The bioengineered scaffold containing ASCs induced thick epithelium, and then pseudostratified epithelium containing goblet cells was formed. Furthermore, the application of both GFBs and ASCs had synergistic effects on tracheal epithelial regeneration, suggesting that bioengineered scaffolds containing GFBs and ASCs are useful for hastening tracheal epithelial regeneration.
在某些类型的气管疾病中，需要进行气管切除术。对于接受气管切除术的患者，我们小组已经在临床上使用了人工移植物，该移植物由带有螺旋状聚丙烯支架和网眼的胶原海绵制成。然而，上皮细胞的再生被证实是缓慢的。在本研究中，我们研究了牙龈成纤维细胞（GFB）和脂肪源性干细胞（ASC）作为自体移植细胞与人工移植物结合用于气管上皮再生的潜力。在与气管上皮细胞的体外共培养中，GFB 刺激上皮细胞分化和假复层上皮的重建。ASC 刺激上皮细胞增殖和多层上皮的重建。随后，我们用 GFB 和/或 ASC 制备了三种生物工程支架，并将其植入大鼠气管缺损处。含有 GFB 的生物工程支架在移植后 1 周内被气管上皮细胞覆盖，2 周后形成高纤毛上皮。含有 ASC 的生物工程支架诱导了厚的上皮，然后形成含有杯状细胞的假复层上皮。此外，GFB 和 ASC 的联合应用对气管上皮再生具有协同作用，这表明含有 GFB 和 ASC 的生物工程支架有助于加速气管上皮再生。
