脂质耗竭对膜钠钾离子依赖性三磷酸腺苷酶和钾离子依赖性磷酸酶的不同影响。
Differential effects of lipid depletion on membrane sodium-plus-potassium ion-dependent adenosine triphosphatase and potassium ion-dependent phosphatase.
作者信息
Wheeler K P, Walker J A
出版信息
Biochem J. 1975 Mar;146(3):723-7. doi: 10.1042/bj1460723.
The phospholipid-dependence of the (Na-++K-+)-dependent ATPase (adenosine triphosphatase) (EC 3.6.1.3) and associated K-+-dependent phosphatase activity (EC 3.6.1.7) have been compared. Unlike the (Na-++K-+)-dependent ATPase activities, the K-+-dependent phosphatase activities of a number of different preparations were not closely correlated with their total phospholipid contents. After partial lipid depletion with a single extraction in Lubrol W the residual ATPase and phosphatase activities were correlated, but their magnitudes were quite different: on average only about 5% of the former remained compared with 50% of the latter. A similar differential effect on these activities was found after extraction with deoxycholate. In contrast with the ATPase, consistent restoration of the phosphatase activity of Lubrol-extracted enzymes by added exogenous phospholipids was not observed. We conclude that, although the K-+-dependent phosphatase may be lipid-dependent, the lipid requirement must be different from that of the complete ATPase system, and this difference should help investigations of their relationship.
对（Na⁺+K⁺）依赖的ATP酶（腺苷三磷酸酶）（EC 3.6.1.3）以及相关的K⁺依赖的磷酸酶活性（EC 3.6.1.7）的磷脂依赖性进行了比较。与（Na⁺+K⁺）依赖的ATP酶活性不同，许多不同制剂的K⁺依赖的磷酸酶活性与其总磷脂含量没有密切相关性。用Lubrol W单次提取进行部分脂质去除后，残留的ATP酶和磷酸酶活性具有相关性，但它们的大小差异很大：前者平均仅保留约5%，而后者保留50%。用脱氧胆酸盐提取后，对这些活性也发现了类似的差异效应。与ATP酶相反，未观察到通过添加外源性磷脂使Lubrol提取的酶的磷酸酶活性持续恢复。我们得出结论，虽然K⁺依赖的磷酸酶可能依赖脂质，但其脂质需求一定不同于完整的ATP酶系统，这种差异应有助于对它们之间关系的研究。
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