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J Zhejiang Univ Sci B. 2006 Jul;7(7):591-5. doi: 10.1631/jzus.2006.B0591.
2
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Hormone-resistant mutants of Arabidopsis have an attenuated response to agrobacterium strains.拟南芥激素抗性突变体对农杆菌菌株的反应减弱。
Plant Physiol. 1992 Mar;98(3):979-83. doi: 10.1104/pp.98.3.979.
2
Loss-of-function mutations in the ethylene receptor ETR1 cause enhanced sensitivity and exaggerated response to ethylene in Arabidopsis.乙烯受体ETR1中的功能丧失突变导致拟南芥对乙烯的敏感性增强和反应过度。
Plant Physiol. 2002 Aug;129(4):1557-67. doi: 10.1104/pp.003780.
3
The tomato ethylene receptor gene family: Form and function.
Physiol Plant. 2002 Jul;115(3):336-341. doi: 10.1034/j.1399-3054.2002.1150302.x.
4
Delayed abscission and shorter Internodes correlate with a reduction in the ethylene receptor LeETR1 transcript in transgenic tomato.转基因番茄中乙烯受体LeETR1转录本的减少与脱落延迟和节间缩短相关。
Plant Physiol. 2002 Mar;128(3):978-87. doi: 10.1104/pp.010782.
5
Ethylene hormone receptor action in Arabidopsis.拟南芥中的乙烯激素受体作用
Bioessays. 2001 Jul;23(7):619-27. doi: 10.1002/bies.1087.
6
Reduced expression of the tomato ethylene receptor gene LeETR4 enhances the hypersensitive response to Xanthomonas campestris pv. vesicatoria.番茄乙烯受体基因LeETR4的表达降低增强了对野油菜黄单胞菌疮痂致病变种的过敏反应。
Mol Plant Microbe Interact. 2001 Apr;14(4):487-95. doi: 10.1094/MPMI.2001.14.4.487.
7
Ethylene: a gaseous signal molecule in plants.乙烯：植物中的一种气态信号分子。
Annu Rev Cell Dev Biol. 2000;16:1-18. doi: 10.1146/annurev.cellbio.16.1.1.
8
Response to Xanthomonas campestris pv. vesicatoria in tomato involves regulation of ethylene receptor gene expression.番茄对野油菜黄单胞菌疮痂致病变种的反应涉及乙烯受体基因表达的调控。
Plant Physiol. 2000 May;123(1):81-92. doi: 10.1104/pp.123.1.81.
9
The tomato ethylene receptors NR and LeETR4 are negative regulators of ethylene response and exhibit functional compensation within a multigene family.番茄乙烯受体NR和LeETR4是乙烯反应的负调控因子，在多基因家族中表现出功能补偿。
Proc Natl Acad Sci U S A. 2000 May 9;97(10):5663-8. doi: 10.1073/pnas.090550597.
10
Differential expression of two novel members of the tomato ethylene-receptor family.番茄乙烯受体家族两个新成员的差异表达
Plant Physiol. 1999 May;120(1):165-72. doi: 10.1104/pp.120.1.165.

针对乙烯受体基因LeETR1[校正后]和LeETR2[校正后]进行反义操作的转基因番茄的特性

Characteristics of transgenic tomatoes antisensed for the ethylene receptor genes LeETR1 [corrected] and LeETR2 [corrected].

作者信息

Wang Zhong-feng, Ying Tie-jin, Zhang Ying, Bao Bi-li, Huang Xiao-dan

机构信息

School of Biosystem Engineering and Food Science, Zhejiang University, Hangzhou 310029, China.

出版信息

J Zhejiang Univ Sci B. 2006 Jul;7(7):591-5. doi: 10.1631/jzus.2006.B0591.

DOI:10.1631/jzus.2006.B0591
PMID:16773735
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1500884/
Abstract

Two stable transformed lines containing antisense LeETR1 [corrected] or LeETR2 [corrected] sequences and their hybridized line were investigated to determine the effect of LeETR1 [corrected] and LeETR2 [corrected] specificity in the ethylene receptor family in tomato (Lycopersicon esculentum Mill.) on ethylene signaling. The transgenic line ale1 containing antisense LeETR1 [corrected] displayed shorter length of seedling grown in the dark and adult plant in the light, severe epinastic petiole, and accelerated abscission of petiole explant and senescence of flower explant, compared with its wild type B1. The transgenic line ale2 containing antisense LeETR2 [corrected] also exhibited shorter hypocotyls and slightly accelerated abscission. The phenotypes of cross line dale of LeETR1 [corrected] and LeETR2 [corrected] were close to ale1 in many aspects. These results suggested that LeETR1 [corrected] probably plays a relatively important role in ethylene signaling of tomato growth and development.

摘要

研究了两个含有反义LeETR1[校正后]或LeETR2[校正后]序列的稳定转化株系及其杂交株系，以确定番茄（Lycopersicon esculentum Mill.）乙烯受体家族中LeETR1[校正后]和LeETR2[校正后]特异性对乙烯信号传导的影响。与野生型B1相比，含有反义LeETR1[校正后]的转基因株系ale1在黑暗中生长的幼苗和光照下的成年植株长度较短，叶柄严重向下弯曲，叶柄外植体脱落加速，花外植体衰老加速。含有反义LeETR2[校正后]的转基因株系ale2也表现出较短的下胚轴和略加速的脱落。LeETR1[校正后]和LeETR2[校正后]的杂交株系dale的表型在许多方面与ale1相似。这些结果表明，LeETR1[校正后]可能在番茄生长发育的乙烯信号传导中起相对重要的作用。