免疫过氧化物酶试验在检测尿液中包被细菌的各类抗体（IgA、IgG和IgM）中的应用
[The application of immunoperoxidase test for the detection of antibodies various classes (IgA, IgG and IgM) coating bacteria in urine].
作者信息
Jakoniuk Piotr, Leszczyńska Katarzyna, Zalewska Marta, Sacha Paweł, Wieczorek Piotr
机构信息
Zakład Diagnostyki Mikrobiologicznej Akademii Medycznej w Białymstoku.
出版信息
Med Dosw Mikrobiol. 2005;57(4):431-6.
For the detection of bacteria coated with immunoglobulins in urine the monoclonal antibodies against human IgA, IgG and IgM conjugated with peroxidase were used. For comparison, the immunofluorescence technique was also employed. The results obtained by two methods revealed that immunofluorescence were less sensitive. It was found that bacteria were predominantly coated with IgA (41,9 +/- 22,4%) and IgG (34,1 +/- 15,3 %) immunoglobulins. The IgM antibodies were found rarely (12,8 +/- 8%).
为检测尿液中包被免疫球蛋白的细菌，使用了与过氧化物酶偶联的抗人IgA、IgG和IgM单克隆抗体。作为对照，也采用了免疫荧光技术。两种方法所得结果显示，免疫荧光法敏感性较低。发现细菌主要包被有IgA（41.9±22.4%）和IgG（34.1±15.3%）免疫球蛋白。IgM抗体很少被发现（12.8±8%）。
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