Hatziefthimiou A, Mitsacos A, Mitsaki E, Plaitakis A, Kouvelas E D
Department of Physiology, Medical School, University of Patras, Greece.
J Neurosci Res. 1991 Mar;28(3):367-75. doi: 10.1002/jnr.490280308.
In the present work the distribution of L-glutamate binding sites in the different layers of human cerebellum of normal individuals and of seven patients who died with olivopontocerebellar atrophy (OPCA) was examined with the technique of quantitative autoradiography. Specific L-[3H]glutamate binding was higher in the molecular than in the granule cell layer of normal cerebellar tissue. A significant decrease of L-[3H]glutamate specific binding was observed in the molecular layer of all OPCA tissues. In the granule cell layer L-[3H]glutamate binding was decreased only in two patients who suffered from late-onset sporadic OPCA and in one patient who suffered from a form of OPCA inherited in a dominant manner. Quisqualate-sensitive binding sites were the most abundant binding sites in the molecular layer of normal cerebella, whereas N-methyl-D-aspartic acid (NMDA)-sensitive binding sites were the most abundant type in the granule cell layer. A significant decrease of quisqualate-sensitive and an increase in NMDA-sensitive binding sites were observed in the molecular layer of OPCA cerebellar tissues. No significant changes were observed in the granule cell layer of these tissues.
在本研究中，运用定量放射自显影技术，检测了正常个体以及7例死于橄榄体脑桥小脑萎缩（OPCA）患者的人类小脑不同层中L-谷氨酸结合位点的分布情况。在正常小脑组织中，分子层的特异性L-[3H]谷氨酸结合高于颗粒细胞层。在所有OPCA组织的分子层中，均观察到L-[3H]谷氨酸特异性结合显著降低。在颗粒细胞层，仅在2例迟发性散发性OPCA患者和1例显性遗传形式的OPCA患者中，L-[3H]谷氨酸结合降低。在正常小脑的分子层中，对quisqualate敏感的结合位点是最丰富的结合位点，而在颗粒细胞层中，对N-甲基-D-天冬氨酸（NMDA）敏感的结合位点是最丰富的类型。在OPCA小脑组织的分子层中，观察到对quisqualate敏感的结合位点显著减少，而对NMDA敏感的结合位点增加。在这些组织的颗粒细胞层中未观察到显著变化。
