与水混溶的有机溶剂对溶液中及固定在Eupergit CM上的α-胰凝乳蛋白酶的影响。
Influence of water miscible organic solvents on alpha-chymotrypsin in solution and immobilized on Eupergit CM.
作者信息
Olofsson Linus, Söderberg Pernilla, Nicholls Ian A
机构信息
Department of Chemistry & Biomedical Sciences, University of Kalmar, SE-391 82, Kalmar, Sweden.
出版信息
Biotechnol Lett. 2006 Jun;28(12):929-35. doi: 10.1007/s10529-006-9025-7. Epub 2006 Jun 15.
The effects of the water-miscible organic solvents (methanol, ethanol, 1-propanol, 2-propanol, acetonitrile, N,N'-dimethylformamide and tetrahydrofuran) on the stability and catalytic activity of alpha-chymotrypsin (CT) immobilized on Eupergit CM were studied. Enhanced stabilities and activities were observed both as a consequence of immobilization and the presence of organic solvent, which in combination provide long term (at least 24 h) retention of activity, and up to 50-fold increase in 50% (v/v) methanol in buffer. Low quantities (20%, v/v) of acetonitrile not only prevented CT inactivation by autolysis at 20 degrees C but also induced a significant increase in the activity of both free (six-fold) and immobilized (two-fold) CT.
研究了与水混溶的有机溶剂（甲醇、乙醇、1-丙醇、2-丙醇、乙腈、N，N'-二甲基甲酰胺和四氢呋喃）对固定在Eupergit CM上的α-胰凝乳蛋白酶（CT）稳定性和催化活性的影响。固定化以及有机溶剂的存在均增强了稳定性和活性，二者共同作用可实现活性的长期（至少24小时）保留，且在缓冲液中50%（v/v）甲醇存在时活性可提高至50倍。低含量（20%，v/v）的乙腈不仅可防止CT在20℃下因自溶而失活，还能使游离CT（提高6倍）和固定化CT（提高2倍）的活性显著增加。
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