Gorvel J P, Ferrero A, Chambraud L, Rigal A, Bonicel J, Maroux S
Centre de Biochimie et de Biologie Moléculaire Centre National de la Recherche Scientifique, Marseille, France.
Gastroenterology. 1991 Sep;101(3):618-25. doi: 10.1016/0016-5085(91)90517-o.
One monoclonal antibody (8A9) against the human sucrase-isomaltase complex and one (4H3) against the human dipeptidylpeptidase IV were produced in the rat and used to immunolabel thin frozen sections of human small intestine and colon. Both enzymes were found to be expressed in the poorly differentiated crypt cells of the small intestine as well as in the mature villous cells, and very low levels were found to be expressed in the colon. Homogeneous immunolabeling of the whole colonic epithelium with the monoclonal antibody 4H3 was often observed, whereas labeling with the monoclonal antibody 8A9, if any, was either restricted to a few crypts and plateaus. The two antibodies were used to perform specific immunoprecipitation of the corresponding antigen, the N-terminal sequence of which was determined after sodium dodecyl sulfate-polyacrylamide gel electrophoresis purification and electroblotting, and were compared with those of other species. In secretor blood group A humans, both the sucrase-isomaltase and the dipeptidylpeptidase IV have type 3 blood group A determinants.
在大鼠体内制备了一种抗人蔗糖酶-异麦芽糖酶复合物的单克隆抗体（8A9）和一种抗人二肽基肽酶IV的单克隆抗体（4H3），并用于对人小肠和结肠的冷冻薄切片进行免疫标记。发现这两种酶在小肠分化差的隐窝细胞以及成熟的绒毛细胞中均有表达，而在结肠中表达水平极低。经常观察到单克隆抗体4H3对整个结肠上皮进行均匀免疫标记，而单克隆抗体8A9的标记（若有）则仅限于少数隐窝和平坦区。这两种抗体用于对相应抗原进行特异性免疫沉淀，在十二烷基硫酸钠-聚丙烯酰胺凝胶电泳纯化和电印迹后确定其N端序列，并与其他物种的序列进行比较。在分泌型A血型的人中，蔗糖酶-异麦芽糖酶和二肽基肽酶IV均具有3型A血型决定簇。
