Miao Zhi-lin, Zeng Ding-yin, Sun Xi-zhuo, Zhou Xu-chen, Cheng Ying, Guan Qi-gang, Zhang Li, He Xue-zhi, Han Feng-tong
Deptment of Cardiovascular Disease, First Affiliated Hospital, China Medical University, Shenyang 110001, China.
Zhonghua Xin Xue Guan Bing Za Zhi. 2006 May;34(5):445-9.
To observe the effects of rapamycin on the expressions of Rho-kinase and p27 mRNA during vascular intimal proliferation in a porcine model of coronary stenosis induced by interleukin-1beta (IL-1beta).
The proximal segments of LAD and LCX were wrapped with cotton mesh that had absorbed sepharose bead solution with or without IL-1beta. Selective coronary angiography was performed two weeks later and the animals were killed for collecting the samples for histopathology and RT-PCR analyzing of Rho-kinase and p27 mRNA.
The expressions of Rho-kinase and p27 mRNA could be visualized in normal coronary wall. The expression of Rho-kinase mRNA was significantly enhanced and the expression of p27 mRNA was significantly decreased during the process of intimal proliferation induced by IL-1beta. Rapamycin significantly inhibited the intimal proliferation, reduced the infiltration of inflammatory cells, reduced the expression of Rho-kinase mRNA and increased the expression of p27 mRNA.
The expression of Rho-kinase mRNA is upregulated and p27 mRNA downregulated in coronary artery stenosis induced by IL-1beta and these effects could be abolished by cotreatment with rapamycin.
观察雷帕霉素对白细胞介素-1β（IL-1β）诱导的猪冠状动脉狭窄模型血管内膜增殖过程中Rho激酶和p27 mRNA表达的影响。
用吸附有含或不含IL-1β的琼脂糖珠溶液的棉网包裹左前降支（LAD）和左旋支（LCX）的近端节段。两周后进行选择性冠状动脉造影，然后处死动物，收集样本进行组织病理学检查以及Rho激酶和p27 mRNA的逆转录-聚合酶链反应（RT-PCR）分析。
在正常冠状动脉壁中可观察到Rho激酶和p27 mRNA的表达。在IL-1β诱导的内膜增殖过程中，Rho激酶mRNA的表达显著增强，而p27 mRNA的表达显著降低。雷帕霉素显著抑制内膜增殖，减少炎性细胞浸润，降低Rho激酶mRNA的表达并增加p27 mRNA的表达。
在IL-1β诱导的冠状动脉狭窄中，Rho激酶mRNA表达上调，p27 mRNA表达下调，而雷帕霉素联合治疗可消除这些影响。
