[Inhibition of the expression of p42MAPK in HeLa cell line by RNA interference].
作者信息
Huang Chen, Liu Li-ying, Song Tu-sheng, Ni Lei, Song Li-ping, Hu Jing-song, Zhao Xiao-ge, Si Lü-sheng
机构信息
Key Laboratory of Environment and Gene Related to Diseases, Ministry of Education, Medical School of Xi'an Jiaotong University, Xi'an 710061, China.
出版信息
Zhonghua Bing Li Xue Za Zhi. 2006 May;35(5):292-5.
OBJECTIVE
To screen for siRNAs that inhibit the expression of p42(MAPK) in HeLa cell line.
METHODS
Three p42(MAPK) siRNAs and one random siRNA were synthesized using Silencer siRNA Construction Kit, and labeled with Cy-3 for measurement of transfection effect. SiRNAs were transfected into HeLa cells by Lipofectamin 2000. The expression of p42(MAPK) was analyzed by Western blot. The biological effect of siRNAs on HeLa cell growth was monitored by MTT and flow cytometry.
RESULTS
Two siRNAs (siRNA-2 and siRNA-3) among three tested were identified to be able to downregulate the p42(MAPK) expression. A concurrent growth retardation of HeLa cell line was observed in comparison with the control.
CONCLUSION
Inhibition of p42(MAPK) expression with siRNA technique can inhibit the proliferation of HeLa cells.
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