You Peng, Liu Yu-lan, Chen Guo-dong
Department of Gastroenterology, Peking University People's Hospital, Beijing 100044, China.
Beijing Da Xue Xue Bao Yi Xue Ban. 2006 Jun 18;38(3):266-70.
To explore the roles of CD4+ CD25+ and CD8+ CD28- T regulatory cells in spontaneous tolerance of inbred rats orthotopic liver Transplantation.
The orthotopic liver transplantation models of spontaneous tolerance were established between BN (donor) and LEW (recipient) inbred rats whose survival time was more than 120 days, The expression of CD8+ CD28- T and CD4+ CD25+ T cells in PBMC and spleen were determined by flow cytometry. CD8+ CD28- T and CD4+ CD25+ T cells were sorted by immunomagnetic beads. The expressions of CD25, CD28, CD152 and CD45RO on CD8+ CD28- T and CD4+ CD25+ T regulatory cells were also determined by flow cytometry.
Compared with control groups, the proportion of CD8+ CD28- T regulatory cells in spontaneous models was significantly increased in peripheral blood mononuclear cells (PBMC) and spleen [PBMC (30.2+/-5.8)% vs (16.4+/-6.1)%; Spleen (23.7+/-7.2)% vs(13.4+/-5.5)% P<0.01]. CD4+ CD25+ T regulatory cells had no change; CD11b was expressed on CD8+ CD28- T regulatory cells in both control groups and tolerance groups, and CD25, CD152 (CTL-4) were not expressed on CD8+ CD28- T regulatory cells. The expression of CD45RO on CD8+ CD28- T regulatory cells was 76.1% in control groups and more than 90% in tolerance groups; CD11b, CD152 (CTL-4) and CD45RO were expressed on CD4+ CD25+ T regulatory cells in both control groups and tolerance groups.
The roles of CD8+ CD28- T cells were more important than CD4+ CD25+ T cells in spontaneous tolerance of rat liver transplantation. the different expressions of CD152 showed the functions of CD8+ CD28- and CD4+ CD25+ T cells were different. The different expressions of CD45RO suggested CD8+ CD28- T regulatory cells was changed before and after liver transplantation.
探讨CD4+CD25+和CD8+CD28-T调节性细胞在近交系大鼠原位肝移植自发免疫耐受中的作用。
建立BN（供体）和LEW（受体）近交系大鼠原位肝移植自发免疫耐受模型，其存活时间超过120天，采用流式细胞术检测外周血单个核细胞（PBMC）和脾脏中CD8+CD28-T和CD4+CD25+T细胞的表达。通过免疫磁珠分选CD8+CD28-T和CD4+CD25+T细胞。采用流式细胞术检测CD8+CD28-T和CD4+CD25+调节性细胞上CD25、CD28、CD152和CD45RO的表达。
与对照组相比，自发免疫耐受模型外周血单个核细胞（PBMC）和脾脏中CD8+CD28-T调节性细胞比例显著升高[PBMC（30.2±5.8）%比（16.4±6.1）%；脾脏（23.7±7.2）%比（13.4±5.5）%，P<0.01]。CD4+CD25+T调节性细胞无变化；对照组和免疫耐受组CD8+CD28-T调节性细胞均表达CD11b，CD8+CD28-T调节性细胞不表达CD25、CD152（CTLA-4）。对照组CD8+CD28-T调节性细胞上CD45RO表达为76.1%，免疫耐受组超过90%；对照组和免疫耐受组CD4+CD25+T调节性细胞均表达CD11b、CD152（CTLA-4）和CD45RO。
在大鼠肝移植自发免疫耐受中，CD8+CD28-T细胞的作用比CD4+CD25+T细胞更重要。CD152的不同表达显示CD8+CD28-和CD4+CD25+T细胞功能不同。CD45RO的不同表达提示肝移植前后CD8+CD28-T调节性细胞发生了变化。
