Fessenmaier M, Frank R, Retey J, Schubert C
Institute of Organic Chemistry, University of Karlsruhe, Germany.
FEBS Lett. 1991 Jul 29;286(1-2):55-7. doi: 10.1016/0014-5793(91)80938-y.
A clone harbouring the entire urocanase gene (hutU) was obtained from a genomic library of Pseudomonas putida using oligonucleotide probes synthesised on the basis of known flanking sequences. One subunit of urocanase consists of 556 amino acids and has a molecular mass of 60,771 Da.