Dong Yuzhi, Denny Thomas N
Center for Laboratory Investigation, Department of Pathology, Laboratory Medicine, and Pediatrics, New Jersey Medical School, University of Medicine and Dentistry of New Jersey, Newark, Newark, NJ 07103, USA.
J Infect Dis. 2006 Jul 15;194(2):168-75. doi: 10.1086/505224. Epub 2006 Jun 12.
Classical major histocompatibility complex class I-restricted CD8(+) cytotoxic T lymphocytes (CTLs) play an important role in protective immunity against infection with smallpox virus. The identification of target antigens is crucial for defining the role played by CD8(+) CTL responses in host resistance to smallpox and for the design of vaccines that produce effective cell-based responses. We report the identification of a novel human leukocyte antigen-A*0201-restricted epitope (J8R(11-19)) within A55R of vaccinia virus (VV) that is conserved in J8R of smallpox virus variola major. The J8R(11-19)-specific CTL line and CTL clone exerted physiologically relevant functions as they recognized VV-infected lymphoblastoid JY cells or autologous B lymphoblastoid cell lines, and the cytolytic activity was accompanied by the production of interferon- gamma , tumor necrosis factor- alpha , and interleukin-2. The CTL response was detected in individuals who had been vaccinated >30 years ago or had recently received a booster of current smallpox vaccine (Dryvax) but not in VV-naive donors.
经典的主要组织相容性复合体I类限制性CD8(+)细胞毒性T淋巴细胞（CTL）在针对天花病毒感染的保护性免疫中发挥重要作用。鉴定靶抗原对于确定CD8(+) CTL反应在宿主抵抗天花中的作用以及设计能产生有效细胞介导反应的疫苗至关重要。我们报告了在痘苗病毒（VV）的A55R内鉴定出一种新型的人类白细胞抗原-A*0201限制性表位（J8R(11-19)），该表位在天花病毒大天花的J8R中保守。J8R(11-19)特异性CTL系和CTL克隆发挥了生理相关功能，因为它们识别VV感染的淋巴母细胞样JY细胞或自体B淋巴母细胞样细胞系，并且细胞溶解活性伴随着干扰素-γ、肿瘤坏死因子-α和白细胞介素-2的产生。在30多年前接种过疫苗或最近接受过当前天花疫苗（Dryvax）加强免疫的个体中检测到了CTL反应，但在未接触过VV的供体中未检测到。
