Gardner Erin R, Liau Chi-Ting, Chu Zyting E, Figg William D, Sparreboom Alex
Clinical Pharmacology Research Core, SAIC-Frederick, Inc., NCI-Frederick, Frederick, MD 21702, USA.
Rapid Commun Mass Spectrom. 2006;20(14):2170-4. doi: 10.1002/rcm.2577.
A sensitive and specific assay for paclitaxel in plasma has been developed to overcome limitations in previously published assays, using liquid chromatography with tandem mass spectrometric detection. Plasma samples (100 microL) were subjected to liquid-liquid extraction with 1-chlorobutane/acetonitrile (4:1, v/v), with [(2)H(5)]paclitaxel employed as the internal standard. Chromatography was carried out with a Waters SymmetryShield C8 column (50 x 2.1 mm, 3.5 microm). The total run time, including equilibration, was 8 min, using a gradient of acetonitrile and 10 mM ammonium formate, pH 4.0. The assay is accurate and precise over the range of 2-2500 ng/mL and has been successfully applied to study the clinical pharmacokinetics of two formulations of paclitaxel, Genaxol and Genetaxyl, given orally and intravenously.
为克服先前发表的检测方法的局限性，已开发出一种灵敏且特异的血浆中紫杉醇检测方法，该方法采用液相色谱-串联质谱检测。取100微升血浆样品，用1-氯丁烷/乙腈（4:1，v/v）进行液-液萃取，以[(2)H(5)]紫杉醇作为内标。使用沃特世SymmetryShield C8柱（50×2.1毫米，3.5微米）进行色谱分析。包括平衡时间在内的总运行时间为8分钟，采用乙腈和10 mM甲酸铵（pH 4.0）的梯度洗脱。该检测方法在2-2500 ng/mL范围内准确且精密，已成功应用于研究口服和静脉注射两种紫杉醇制剂（Genaxol和Genetaxyl）的临床药代动力学。
