Ali Khan Mohd Wajid, Ahmad Masood
Department of Biochemistry, Faculty of Life Sciences, Aligarh Muslim University, Aligarh, India.
J Environ Sci Health A Tox Hazard Subst Environ Eng. 2006;41(4):659-74. doi: 10.1080/10934520600575051.
A Pseudomonas fluorescens strain was isolated from the soil of industrial estate of Aligarh, India. This strain was resistant to some of the major Indian water pollutants, namely Cd2+, Cr6+, Cu2+, Ni2+, Pb2+, BHC, 2,4-D, mancozeb and phenols up to the levels occurring in the highly polluted regions. Moreover, the test strain seems to have a great potential for the detoxification of these pollutants. The decrease in toxicity as determined by the Allium cepa test was recorded as 62.5% for the model water containing the mixture of test heavy metals, 71.9% for the pesticides, 73.2% for phenols, and 58.5% for combination of all these toxicants. These values were obtained after 24 hours, exposure to the immobilized cells of the test isolate in the calcium alginate matrix at the concentrations of these polutants supposedly present in the highly polluted water systems in India. The efficiency of bioremediation for certain heavy metals at the same concentrations by means of immobilized cells of the test Pseudomonas fluorescens isolate was estimated to be 75.9% for cadmium, 74.2% for hexavalent chromium and 61.0% for lead during the 24 hours' treatment. In view of the preliminary work, the test isolate seems to be a good candidate for the bioremediation of water pollutants.
从印度阿里格尔工业区的土壤中分离出了一株荧光假单胞菌。该菌株对印度一些主要的水体污染物具有抗性，这些污染物包括Cd2+、Cr6+、Cu2+、Ni2+、Pb2+、六六六、2,4-滴、代森锰锌和酚类，其抗性水平可达高污染地区出现的浓度。此外，该测试菌株似乎在这些污染物的解毒方面具有很大潜力。通过洋葱根尖试验测定，含有测试重金属混合物的模拟水中毒性降低了62.5%，农药降低了71.9%，酚类降低了73.2%，所有这些有毒物质的混合物降低了58.5%。这些数值是在将测试分离株的固定化细胞暴露于藻酸钙基质中24小时后获得的，其浓度为印度高污染水系统中可能存在的这些污染物的浓度。在24小时的处理过程中，通过测试荧光假单胞菌分离株的固定化细胞对相同浓度的某些重金属的生物修复效率估计为：镉75.9%，六价铬74.2%，铅61.0%。鉴于初步工作，该测试分离株似乎是水体污染物生物修复的一个良好候选菌株。
