Alonso A, Almendral M J, Porras M J, Curto Y
Departamento de Química Analítica, Nutrición y Bromatología, Faculty of Chemistry, University of Salamanca, Plaza de la Merced s/n, E-37008 Salamanca, Spain.
J Pharm Biomed Anal. 2006 Sep 18;42(2):171-7. doi: 10.1016/j.jpba.2006.02.011. Epub 2006 Jun 15.
Two modes of liquid-liquid extraction in flow-injection systems were compared and applied to the fluorimetric determination of thiamine (Vitamin B(1)). The first included phase segmentation, but fluorescence was measured without phase separation. In this mode, thiamine was detected at concentrations higher than 8 microg/l with a linear application range of 30-2,000 microg/l, an R.S.D. of 1.9% (150 microg/l, n=10) and a sampling frequency of 60/h. In the second mode, a single segment of organic solution was injected into the aqueous stream and fluorescence was also measured without phase separation. Using this mode, concentrations of thiamine higher than 1 microg/l were detected, with a linear application range between 5 and 280 microg/l, an R.S.D. of 2.4% (150 microg/l, n=10) and a sampling frequency of 60/h. The two forms were applied to the analysis of thiamine in pharmaceuticals.
对流动注射系统中两种液-液萃取模式进行了比较，并将其应用于硫胺素（维生素B1）的荧光测定。第一种模式包括相分割，但在不进行相分离的情况下测量荧光。在此模式下，硫胺素在浓度高于8μg/L时被检测到，线性应用范围为30-2000μg/L，相对标准偏差为1.9%（150μg/L，n=10），采样频率为60次/小时。在第二种模式中，将一段有机溶液注入水流中，同样在不进行相分离的情况下测量荧光。使用这种模式，检测到硫胺素浓度高于1μg/L，线性应用范围在5-280μg/L之间，相对标准偏差为2.4%（150μg/L，n=10），采样频率为60次/小时。这两种模式被应用于药物中硫胺素的分析。
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