通过瞬态电双折射对蛋白质 - 十二烷基硫酸钠复合物的研究。

A study of protein-sodium dodecyl sulfate complexes by transient electric birefringence.

作者信息

Wright A K, Thompson M R, Miller R L

出版信息

Biochemistry. 1975 Jul 15;14(14):3224-8. doi: 10.1021/bi00685a030.

Abstract

The method of transient electric birefringence has been applied to study the conformation of protein-sodium dodecyl sulfate complexes. A model of a deformable prolate ellipsoid has been proposed for the protein-dodecyl sulfate complex. This model is compared to the models proposed by J. A. Reynolds and C. Tanford (1970), J. Biol. Chem. 245, 5161) and K. Shirahama, K. Tsujii, and T. Takagi (1974, J. Biochem. 75, 309). Differences between these latter two models are resolved by the model presented here. In addition, it has been demonstrated that protein molecular weights may be obtained from the slow relaxation time for transient electric birefringence of protein-dodecyl sulfate complexes.

摘要

瞬态电双折射方法已被用于研究蛋白质 - 十二烷基硫酸钠复合物的构象。已提出一种可变形长椭球体模型用于蛋白质 - 十二烷基硫酸盐复合物。该模型与J. A. 雷诺兹和C. 坦福德（1970年，《生物化学杂志》245, 5161）以及K. 白滨、K. 辻井和T. 高木（1974年，《生物化学杂志》75, 309）提出的模型进行了比较。后两种模型之间的差异通过此处提出的模型得到了解决。此外，已经证明可以从蛋白质 - 十二烷基硫酸盐复合物瞬态电双折射的慢弛豫时间获得蛋白质分子量。