Wang Yemin, Wang Jing, Li Gang
Department of Dermatology and Skin Science, Jack Bell Research Centre, Vancouver Coastal Health Research Institute, University of British Columbia, 2660 Oak Street, Vancouver, BC, Canada V6H 3Z6.
FEBS Lett. 2006 Jul 10;580(16):3787-93. doi: 10.1016/j.febslet.2006.05.065. Epub 2006 Jun 9.
The plant homodomain (PHD) of ING2 was shown to regulate p53-dependent apoptosis through phosphoinositides signaling. However, the role of a predicted leucine zipper-like (LZL) motif in N-terminus of ING2 is unclear. Here, we show that LZL motif is critical for the proper functions of ING2 in DNA repair, apoptosis and chromatin remodeling after UV irradiation. Deletion of LZL domain also abrogated the association between ING2 and p53, but not between ING2 and p300, suggesting that ING2 modulates p53-dependent chromatin remodeling, apoptosis and DNA repair by functioning as a scaffold protein to mediate the interaction between p53 and p300.
ING2的植物同源结构域（PHD）已被证明可通过磷酸肌醇信号传导调节p53依赖性凋亡。然而，ING2 N端预测的亮氨酸拉链样（LZL）基序的作用尚不清楚。在此，我们表明LZL基序对于ING2在紫外线照射后的DNA修复、凋亡和染色质重塑中的正常功能至关重要。LZL结构域的缺失也消除了ING2与p53之间的关联，但不影响ING2与p300之间的关联，这表明ING2通过作为支架蛋白介导p53与p300之间的相互作用来调节p53依赖性染色质重塑、凋亡和DNA修复。
