Calli B, Durmaz S, Mertoglu B
Department of Environmental Engineering, Faculty of Engineering, Marmara University, 34722 Goztepe, Istanbul, Turkey.
Water Sci Technol. 2006;53(8):139-47. doi: 10.2166/wst.2006.244.
To identify the microbial communities in Istanbul, Odayeri Municipal Solid Waste Landfill, leachate samples were collected from different sections at different stabilization phases. In identification of microbial communities in leachate samples, molecular techniques such as FISH, DGGE and cloning based on 16S rRNA and mcrA genes were used. As the chemical and microbiological compositions of the samples were compared, obvious correlations were found between the stability of the landfill section and abundance of active methanogens. On the other hand, there were considerable differences between acidogenic and mature leachate samples in DGGE profiles of archaeal and bacterial 16S rRNA genes. Moreover, in acidogenic leachate samples having BOD5/COD ratio of about 0.5 acetate utilizing Methanosarcina and Methanosaeta species were intensively detected in FISH. Although only very few H2-utilizing methanogens were identified with FISH analysis, most of the clones isolated from mature leachate samples clustered within H2-utilizing Methanobacteriales and Methanomicrobiales according to phylogenetic analysis of 16S rRNA and mcrA clones, respectively.
为了鉴定伊斯坦布尔奥代耶里市固体废弃物填埋场中的微生物群落，在不同稳定阶段从不同区域采集了渗滤液样本。在渗滤液样本微生物群落的鉴定中，使用了基于16S rRNA和mcrA基因的荧光原位杂交（FISH）、变性梯度凝胶电泳（DGGE）和克隆等分子技术。在比较样本的化学和微生物组成时，发现填埋场区域的稳定性与活性产甲烷菌的丰度之间存在明显的相关性。另一方面，产酸渗滤液样本和成熟渗滤液样本在古菌和细菌16S rRNA基因的DGGE图谱上存在显著差异。此外，在生化需氧量与化学需氧量（BOD5/COD）比值约为0.5的产酸渗滤液样本中，利用荧光原位杂交法检测到大量利用乙酸盐的甲烷八叠球菌属和甲烷丝状菌属。虽然通过荧光原位杂交分析仅鉴定出极少数利用氢气的产甲烷菌，但根据16S rRNA和mcrA克隆的系统发育分析，从成熟渗滤液样本中分离出的大多数克隆分别聚集在利用氢气的甲烷杆菌目和甲烷微菌目中。
