[菌斑固体、变形链球菌和葡聚糖的缓冲作用]
[Buffering effects of plaque solid, Streptococcus mutans and glucans].
作者信息
Yue Lin, Bao Xu-dong, Gao Xue-jun
机构信息
Department of Operative Dentistry and Endodontics, Peking University School and Hospital of Stomatology, Beijing 100081, China.
出版信息
Zhonghua Kou Qiang Yi Xue Za Zhi. 2006 May;41(5):263-5.
OBJECTIVE
To investigate the buffer capacity of insoluble plaque mass including bacteria and glucans matrix.
METHODS
Plaque samples (starved or incubated 1 h with 10% sucrose), Streptococcus mutans (S. mutans) mass, and insoluble/soluble glucans were titrated with 1 mmol/L HCl. Meanwhile, the density of bacteria was counted and related to buffer capacity.
RESULTS
The bacteria density had significant effects on acid buffer capacity in dental plaque, but the effect reduced with consuming sugar. S. mutans mass with no sucrose incubating contributed to the buffer capacity of (0.609 +/- 0.202) mmol/L, and S. mutans mass with 2% sucrose had only the buffer capacity of (0.099 +/- 0.047) mmol/L. In the mean time, glucans had no effect on acid buffering (0.028 - 0.032 mmol/L).
CONCLUSIONS
The bacteria including S. mutans may be the main contributor to acid buffering in plaque solid.
目的
研究包括细菌和葡聚糖基质在内的不溶性菌斑块的缓冲能力。
方法
用1 mmol/L盐酸滴定菌斑样本（饥饿状态或用10%蔗糖孵育1小时）、变形链球菌菌量以及不溶性/可溶性葡聚糖。同时，对细菌密度进行计数并与缓冲能力相关联。
结果
细菌密度对牙菌斑中的酸缓冲能力有显著影响，但随着糖的消耗，这种影响会降低。未用蔗糖孵育的变形链球菌菌量对缓冲能力的贡献为（0.609±0.202）mmol/L，而用2%蔗糖孵育的变形链球菌菌量的缓冲能力仅为（0.099±0.047）mmol/L。同时，葡聚糖对酸缓冲没有影响（0.028 - 0.032 mmol/L）。
结论
包括变形链球菌在内的细菌可能是菌斑固体中酸缓冲的主要贡献者。
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