Zou Jing, Shang Ran, Zhou Xue-dong
Department of Pediatric Dentistry, West China School of Stomatology, Sichuan University, Chengdu 610041, China.
Zhonghua Kou Qiang Yi Xue Za Zhi. 2006 May;41(5):269-71.
To find out different transmission ways of oral mutans Streptococci (MS) in nursery children.
The study group included 44 nursery children between 3 and 4 years of age and 20 mothers. Dental plaque samples were collected with sterile toothpick and cultured on MSB plates for 48 h. Individual MS colonies representative of the colonial morphologies were subcultured on TPY plates. These strains were biochemically identified to species level. AP-PCR fingerprinting analysis was preformed after identification.
MS was isolated in oral cavities of 65.9% children in 44 babies and 50% pairs in 20 mother-child pairs. A total of 98 MS isolates from 44 children and 20 mothers were isolated. Thirty-two different amplitype were identified in 10 mother-child pairs colonized by MS and there were similar genotypes in 7 pairs of mother-child. Twenty-nine different amplitype were identified in 24 nursery children, and there were 2 genotypes of MS isolated repeatedly among 13 nursery children.
The presence of matching genotypes of MS among nursery children and their mothers suggests horizontal and vertical transmission.
探究幼儿园儿童口腔变形链球菌（MS）的不同传播途径。
研究组包括44名3至4岁的幼儿园儿童和20名母亲。用无菌牙签采集牙菌斑样本，在MSB平板上培养48小时。选取具有代表性菌落形态的单个MS菌落，转接至TPY平板上进行传代培养。对这些菌株进行生化鉴定至种水平。鉴定后进行AP-PCR指纹图谱分析。
44名婴儿中65.9%的儿童口腔中分离出MS，20对母婴中有50%的母婴对分离出MS。共从44名儿童和20名母亲中分离出98株MS菌株。在10对感染MS的母婴对中鉴定出32种不同的扩增型，7对母婴对中有相似的基因型。在24名幼儿园儿童中鉴定出29种不同的扩增型，13名幼儿园儿童中重复分离出2种MS基因型。
幼儿园儿童与其母亲之间存在MS匹配基因型，提示存在水平传播和垂直传播。
