[脆性组氨酸三联体基因异常甲基化在肝细胞癌患者中的临床病理意义]
[Clinicopathological significance of aberrant methylation of the fragile histidine triad gene in patients with hepatocellular carcinoma].
作者信息
Sun Yun, Geng Xiao-ping, Zhu Li-xin, Xiong Qi-ru, Qian Ye-ben, Dong Gui-yin, Li Xiao-ming
机构信息
Department of General Surgery, the First Affiliated Hospital of Anhui Medical University, Hefei, 230022, China.
出版信息
Zhonghua Wai Ke Za Zhi. 2006 May 1;44(9):609-12.
OBJECTIVE
To investigate the aberrant methylation of fragile histidine triad (FHIT) gene and to explore possible relationship between the aberrant methylation of FHIT and clinicopathological features in hepatocellular carcinoma (HCC).
METHODS
The hypermethylation of FHIT was detected by the methylation specific PCR (MSP) method in 45 patients with HCC (tumoral and nontumoral tissue), 14 cases of normal livers and 4 HCC cell lines (SK-Hep-1, Hep-G2, Hep-3B and Huh7). The correlation of FHIT methylation and clinicopathological features was analyzed.
RESULTS
The frequencies of hypermethylation of FHIT in tumoral and nontumoral tissue, normal liver and cell lines were 71.1%, 64.4%, 14.3% and 75.0%, respectively. A significant relation between hypermethylation of FHIT and poor survival was present (P = 0.0430).
CONCLUSIONS
Hypermethylation of FHIT is a frequent and early event in HCC, it might relate to a poor prognosis for patients with HCC.
目的
研究脆性组氨酸三联体（FHIT）基因的异常甲基化情况，并探讨FHIT基因异常甲基化与肝细胞癌（HCC）临床病理特征之间的可能关系。
方法
采用甲基化特异性PCR（MSP）方法检测45例HCC患者（肿瘤组织和非肿瘤组织）、14例正常肝脏组织以及4种HCC细胞系（SK-Hep-1、Hep-G2、Hep-3B和Huh7）中FHIT基因的高甲基化情况。分析FHIT基因甲基化与临床病理特征之间的相关性。
结果
肿瘤组织、非肿瘤组织、正常肝脏组织及细胞系中FHIT基因高甲基化的频率分别为71.1%、64.4%、14.3%和75.0%。FHIT基因高甲基化与患者生存预后不良存在显著相关性（P = 0.0430）。
结论
FHIT基因高甲基化是HCC中常见的早期事件，可能与HCC患者预后不良有关。
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