使用心肌对比超声心动图对心肌梗死后小鼠进行三维灌注成像。
3D perfusion mapping in post-infarct mice using myocardial contrast echocardiography.
作者信息
French Brent A, Li Yinbo, Klibanov Alexander L, Yang Zequan, Hossack John A
机构信息
Department of Biomedical Engineering, University of Virginia, Charlottesville, VA 22908-0759, USA.
出版信息
Ultrasound Med Biol. 2006 Jun;32(6):805-15. doi: 10.1016/j.ultrasmedbio.2006.03.002.
Myocardial contrast echocardiography (MCE) was used to construct three-dimensional maps of perfusion defects in closed-chest mice, with and without myocardial infarction (MI) induced by permanent coronary ligation. Contiguous, short-axis MCE cine images spanning the heart from apex to base were acquired at 1 mm elevations in each mouse. MCE images at each elevation were color-coded to indicate relative perfusion and were compared with postmortem histology. A strong correlation (R > 0.93) in the size of perfused areas was observed between in vivo measurements and the results of conventional ex vivo tissue staining. 3D multislice and 3D surface renderings of perfusion distribution were created and these perfusion maps also matched well with postmortem histology. These methods provide for the noninvasive determination of the total ischemic region placed at risk by coronary occlusion: this is a critical variable in assessing the potential of novel therapeutic agents to reduce MI size in murine models of ischemia/reperfusion injury.
心肌对比超声心动图（MCE）用于构建闭胸小鼠灌注缺损的三维图谱，这些小鼠有或没有因永久性冠状动脉结扎诱导的心肌梗死（MI）。在每只小鼠中，以1毫米的高度采集从心尖到心底的连续短轴MCE电影图像。每个高度的MCE图像用颜色编码以指示相对灌注，并与死后组织学进行比较。在体内测量与传统离体组织染色结果之间观察到灌注区域大小的强相关性（R>0.93）。创建了灌注分布的三维多层和三维表面渲染图，这些灌注图谱也与死后组织学很好地匹配。这些方法提供了对冠状动脉闭塞所导致的处于危险中的总缺血区域的无创测定：这是评估新型治疗剂在缺血/再灌注损伤小鼠模型中减少MI大小潜力的关键变量。
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