Fantin Giancarlo, Giovannini Pier Paolo, Guerrini Alessandra, Maietti Silvia, Medici Alessandro, Pedrini Paola
Dipartimento di Chimica, Università di Ferrara, Via L. Borsari 46, I-44100 Ferrara, Italy.
Biotechnol Lett. 2006 Jun;28(11):805-10. doi: 10.1007/s10529-006-9000-3. Epub 2006 May 23.
Fungi from Amazonian forest soil (Ecuador) and an Italian factory were screened for Baeyer-Villiger (BV) oxidation of bicyclo [3.2.0]hept-2-en-6-one to 2-oxabicyclo[3.3.0]oct-6-en-3-one (Corey's lactone). Isolates of Fusarium sp. and F. solani produced the (+)-(1R,5S)-lactone while isolates of Aspergillus terricola and A. amazonicus afforded the (-)-(1S,5R)-lactone. Highest conversions (85% yield and 70% enantiomeric excess) were obtained with A. amazonicus grown in presence of 2.7 mM titanium dioxide.
对来自厄瓜多尔亚马逊森林土壤和一家意大利工厂的真菌进行了筛选，考察其将双环[3.2.0]庚-2-烯-6-酮氧化为2-氧杂双环[3.3.0]辛-6-烯-3-酮（科里内酯）的拜耳-维利格（BV）氧化反应。镰刀菌属和茄病镰刀菌的分离株产生（+）-（1R，5S）-内酯，而土栖曲霉和亚马逊曲霉的分离株则产生（-）-（1S，5R）-内酯。在2.7 mM二氧化钛存在下培养的亚马逊曲霉获得了最高转化率（85%产率和70%对映体过量）。
