重组人共有干扰素-α突变体在毕赤酵母中的表达与聚集
Expression and aggregation of recombinant human consensus interferon-alpha mutant by Pichia pastoris.
作者信息
Hao Yuyou, Chu Ju, Wang Yonghong, Zhang Siliang, Zhuang Yingping
机构信息
State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, PR China.
出版信息
Biotechnol Lett. 2006 Jun;28(12):905-9. doi: 10.1007/s10529-006-9024-8. Epub 2006 May 31.
A recombinant human consensus interferon-alpha mutant (cIFN) was expressed in Pichia pastoris. The maximum dry cell weight, cIFN concentration and antiviral activity were 160 g l(-1), 1.24 g l(-1) and 4.1 x 10(7) IU ml(-1), respec tively. The cIFN secreted into the medium was in the form of aggregates dominantly by non-covalent interaction and partially by disulphide bond. When the fermentation supernatant was disaggregated with 6 M guanidine hydrochloride, the antiviral activity of cIFN achieved 2.2 x 10(8) IU ml(-1).
一种重组人共有干扰素-α突变体（cIFN）在毕赤酵母中表达。最大干细胞干重、cIFN浓度和抗病毒活性分别为160 g l(-1)、1.24 g l(-1)和4.1×10(7) IU ml(-1)。分泌到培养基中的cIFN主要以非共价相互作用形成聚集体，部分通过二硫键形成聚集体。当用6 M盐酸胍使发酵上清液解聚时，cIFN的抗病毒活性达到2.2×10(8) IU ml(-1)。
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