Hao Yuyou, Chu Ju, Wang Yonghong, Zhang Siliang, Zhuang Yingping
State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, PR China.
Biotechnol Lett. 2006 Jun;28(12):905-9. doi: 10.1007/s10529-006-9024-8. Epub 2006 May 31.
A recombinant human consensus interferon-alpha mutant (cIFN) was expressed in Pichia pastoris. The maximum dry cell weight, cIFN concentration and antiviral activity were 160 g l(-1), 1.24 g l(-1) and 4.1 x 10(7) IU ml(-1), respec tively. The cIFN secreted into the medium was in the form of aggregates dominantly by non-covalent interaction and partially by disulphide bond. When the fermentation supernatant was disaggregated with 6 M guanidine hydrochloride, the antiviral activity of cIFN achieved 2.2 x 10(8) IU ml(-1).
一种重组人共有干扰素-α突变体（cIFN）在毕赤酵母中表达。最大干细胞干重、cIFN浓度和抗病毒活性分别为160 g l(-1)、1.24 g l(-1)和4.1×10(7) IU ml(-1)。分泌到培养基中的cIFN主要以非共价相互作用形成聚集体，部分通过二硫键形成聚集体。当用6 M盐酸胍使发酵上清液解聚时，cIFN的抗病毒活性达到2.2×10(8) IU ml(-1)。
