Rosas-García Ninfa M
Centro de Biotecnología Genómica-IPN, Boulevard del Maestro s/n. Col. Narciso Mendoza, Reynosa, Tamp. CP 88710, Mexico.
Pest Manag Sci. 2006 Sep;62(9):855-61. doi: 10.1002/ps.1245.
Formulations of Bacillus thuringiensis Berliner (Bt) with insecticidal activity against the sugarcane borer, Diatraea saccharalis Fabricius (Lepidoptera: Pyralidae), were developed and tested under laboratory and field conditions. The formulations were prepared using biodegradable polymers such as modified corn starch as an encapsulating agent, gelatin as an adherent, powdered sugarcane as a feeding stimulant and a Bt var. kurstaki GM-34 strain from a non-sugarcane region as the active ingredient. The spore-crystal complex of this strain was mixed at three different concentrations (30, 70 and 100 g kg(-1)) with the other ingredients. The blends were prepared as spray-dried and granular formulations, and then submitted to laboratory tests with two day old larvae of D. saccharalis and field tests in sugarcane crops with natural sugarcane borer infestation. Spray-dried formulations in laboratory bioassays caused mortality near 100% with all three concentrations, and granular formulations caused mortality around 84%. The field tests showed that spray-dried formulations at 70 and 100 g kg(-1) concentrations were as effective as a commercial bioinsectide (Lepinox), while granular formulations were ineffective.
开发了对甘蔗螟虫（Diatraea saccharalis Fabricius，鳞翅目：螟蛾科）具有杀虫活性的苏云金芽孢杆菌（Bt）制剂，并在实验室和田间条件下进行了测试。这些制剂使用可生物降解的聚合物制备，如用改性玉米淀粉作为包囊剂，明胶作为粘着剂，甘蔗粉作为取食刺激剂，并使用来自非甘蔗种植区的苏云金芽孢杆菌库尔斯塔克变种GM - 34菌株作为活性成分。将该菌株的芽孢 - 晶体复合物与其他成分以三种不同浓度（30、70和100 g kg(-1)）混合。将混合物制成喷雾干燥制剂和颗粒制剂，然后用两天龄的甘蔗螟幼虫进行实验室测试，并在自然感染甘蔗螟的甘蔗作物中进行田间测试。在实验室生物测定中，所有三种浓度的喷雾干燥制剂导致的死亡率接近100%，颗粒制剂导致的死亡率约为84%。田间试验表明，浓度为70和100 g kg(-1)的喷雾干燥制剂与商业生物杀虫剂（Lepinox）效果相同，而颗粒制剂无效。
