Histological and cytological study of the bed nuclei of the stria terminalis in adult rat. II. Oval nucleus: extrinsic inputs, cell types, neuropil, and neuronal modules.

The oval nucleus (Ov) of the bed nuclei of the stria terminalis was studied in adult rats. The Ov is composed of 11 neuron types distributed into a shell and a core domain. The stria terminalis, internal capsule, ventral amygdaloid pathway, and medial forebrain bundle are the main sources of afferents to the neuropil of the Ov. The nucleus shell contains abundant intrinsic neurons possibly connected among themselves and with the core by centripetal axon collaterals. Series of intrinsic neurons in the shell, linked with both short-axon and projecting neurons in the core, suggest a centripetal control of projecting neurons. In situ hybidization for vesicular glutamate transporter (VGlu) and glutamic acid decarboxylase (GAD) show numerous GAD-synthesizing neurons and an absence of VGlu-synthesizing neurons. In the electron microscope, the neuropil of the Ov contains axospinous, axoshaft, axosomatic, mixed (i.e., chemical-electrical), and axoaxonic synapses, in order of frequency. Synaptic boutons in apposition with the initial segment, represent an additional axoaxonic interaction. Further neural synchronization of the Ov occurs via gap junctions between somata, soma-dendrite, and possibly by apposition between axon terminals. The putative inputs from the major tracts of the forebrain coupled with the cytological organization of the Ov make it one of the most complex structures of the mammalian brain.