Panula P, Revuelta A V, Cheney D L, Wu J Y, Costa E
J Comp Neurol. 1984 Jan 1;222(1):69-80. doi: 10.1002/cne.902220107.
Antisera against L-glutamate decarboxylase (GAD), the synthesizing enzyme of gamma-aminobutyric acid (GABA) were used to locate GABAergic neurons and nerve terminals in the septal complex of the rat by using the peroxidase-antiperoxidase method. Varying densities of immunoreactive terminals were observed in saline-treated rats but nerve cell bodies were only demonstrated after interventricular or intraseptal injections of colchicine. Small and medium-sized GAD-positive neurons were found in lateral septal nuclei, the largest number of these cells being in the pars dorsalis, and in the bed nucleus of the stria terminalis. Several GAD-immunoreactive neurons were located in the medial septal nucleus and the nucleus of the diagonal band of Broca (DB), where the cells were larger in the ventral than dorsal parts of the region. In the medial septal nucleus and in DB the GAD-positive cell bodies were distributed similarly to cholinergic neurons. Large GAD-positive neurons were also found in the septofimbrial nucleus. Intense immunoreactivity in nerve terminals was observed in the lateral septal nucleus, around the island of Calleja magna, between the DB and nucleus accumbens, and in the septofimbrial and triangular septal nuclei. In contrast, the medial septal nucleus, the DB, and the bed nucleus of the stria terminalis only showed weak to moderate immunoreactivity. These results provide direct morphological evidence for the presence of neurons capable of synthesizing GABA in septal nuclei. We suggest that there are two different GABAergic neuronal systems operating in the septum: a population of small cells in the lateral septal nucleus and a group of large cells in the medial septum and DB.
利用过氧化物酶抗过氧化物酶法，使用抗L-谷氨酸脱羧酶（GAD，γ-氨基丁酸（GABA）的合成酶）抗血清来定位大鼠隔区复合体中的GABA能神经元和神经末梢。在生理盐水处理的大鼠中观察到不同密度的免疫反应性末梢，但仅在脑室内或隔区内注射秋水仙碱后才显示出神经细胞体。在外侧隔核中发现了中小型GAD阳性神经元，其中这些细胞数量最多的位于背侧部，以及终纹床核中。在内侧隔核和布罗卡斜带核（DB）中也发现了几个GAD免疫反应性神经元，该区域腹侧的细胞比背侧的细胞大。在内侧隔核和DB中，GAD阳性细胞体的分布与胆碱能神经元相似。在隔纤维核中也发现了大型GAD阳性神经元。在外侧隔核、大卡列哈岛周围、DB和伏隔核之间以及隔纤维核和三角隔核中观察到神经末梢有强烈的免疫反应性。相比之下，内侧隔核、DB和终纹床核仅显示出弱至中度的免疫反应性。这些结果为隔核中存在能够合成GABA的神经元提供了直接的形态学证据。我们认为在隔区存在两种不同的GABA能神经元系统：外侧隔核中的一群小细胞和内侧隔区及DB中的一群大细胞。
