Meyer H H, Rinke L, Dürsch I
Institut für Physiologie, Süddeutschen Versuchs- und Forschungsanstalt für Milchwirtschaft, Technische Universität München, Freising-Weihenstephan, Germany.
J Chromatogr. 1991 Apr 5;564(2):551-6. doi: 10.1016/0378-4347(91)80523-f.
The antibody for enzyme immunoassay was raised against clenbuterol-diazo-BSA, and salbutamol-carboxymethyl ether-biocytin was used as a label. Procedural blanks from 500 negative urine samples were always less than 0.2 ppb salbutamol or less than 0.02 ppb clenbuterol equivalents, and a residue level of 1 ppb was detected with good reliability. After treatment of veal calves with anabolic dosages, residue levels in urine amounted to 10-200 ppb clenbuterol or salbutamol. beta-Agonists were separated by high-performance liquid chromatography on LiChrospher RP-Select B columns, and acidic methanol-buffer or acetonitrile-buffer mobile phases. Combinations of high-performance liquid chromatography and enzyme immunoassay were used for confirmation.
