Jeanjean R, Hourmant A, Ducet G
Biochimie. 1975;57(3):383-90. doi: 10.1016/s0300-9084(75)80315-4.
The effect of Sulphydryl reagents have been investigated. pCMB inhibits the transport of phosphate in Chlorella pyrenoidosa. This inhibition is immediate and does not change as a function of time of incubation. This inhibition affects non starved and starved cells (phosphate omitted). pCMPS and Mersalyl act in the same manner as pCMB. When these compounds are used at low concentrations, inhibition of phosphate uptake is observed only in starved cells. The substrate (phosphate) cannot provide protection against this inhibition. NEM inhibits phosphate uptake and this inhibition increases as a function of time of incubation. When the time of incubation is very short (about 15 seconds) the effects seems to be superficial and NEM reacts with SH groups involved in the transport system. When phosphate is present (for 15 seconds of incubation with NEM) the inhibition is less important than when phosphate is omitted. The substrate protects against NEM, but this protection disappears as the incubation with NEM is prolonged. NEM inhibits phosphate uptake in non starved and starved cells, however, it is observed that the inhibition is less important in starved cells than in non starved cells. The authors conclude that two kinds of SH groups might exist in the phosphate transport system, one reacting with pCMB and the other with NEM.
已对巯基试剂的作用进行了研究。对氯汞苯甲酸（pCMB）抑制小球藻中磷酸盐的转运。这种抑制作用是即时的，且不会随孵育时间而变化。这种抑制作用对未饥饿和饥饿的细胞（省略磷酸盐）均有影响。对氯苯汞磺酸钠（pCMPS）和汞撒利的作用方式与对氯汞苯甲酸相同。当以低浓度使用这些化合物时，仅在饥饿细胞中观察到对磷酸盐摄取的抑制作用。底物（磷酸盐）无法防止这种抑制作用。N-乙基马来酰亚胺（NEM）抑制磷酸盐摄取，且这种抑制作用随孵育时间而增强。当孵育时间非常短（约15秒）时，这种影响似乎是表面的，且NEM与转运系统中涉及的巯基发生反应。当存在磷酸盐时（与NEM孵育15秒），抑制作用比省略磷酸盐时要小。底物可防止NEM的抑制作用，但随着与NEM孵育时间的延长，这种保护作用会消失。NEM抑制未饥饿和饥饿细胞中磷酸盐的摄取，然而，可以观察到在饥饿细胞中的抑制作用比在未饥饿细胞中要小。作者得出结论，在磷酸盐转运系统中可能存在两种巯基，一种与对氯汞苯甲酸反应，另一种与NEM反应。
