Chen Guang-Fu, Huang Wen-Ge, Chen Feng-Ying, Shan Jin-Lan
Department of Pediatrics, Second People's Hospital of Shenzhen, Shenzhen, Guangdong 518035, China.
Zhongguo Dang Dai Er Ke Za Zhi. 2006 Jun;8(3):239-41.
Trichosanthin (TCS), a ribosome-inactivating protein extracted from the root tuber of Chinese medicinal herb Trichosanthes kirilowii maximowicz, has various pharmacological properties including abortifacient, anti-tumor and anti-virus. This study aimed to evaluate the effects of TCS on infectious brain injury induced by Herpes simplex virus-1 (HSV-1) in mice.
Ninety mice were randomly assigned into three groups: Normal control group (n=30), Model group (n=30) and TCS-treated group (n=30). Viral encephalitis was induced by intracranial inoculation of HSV-1 in the latter two groups. The TCS-treated group was injected with TCS 30 minutes before HSV-1 inoculation. The water content of brain tissue was measured at 1, 12, 24 and 48 hrs, and at 4 and 7 days after HSV-1 inoculation. The viral titer of brain tissue and brain histopathological changes were detected at 7 days after HSV-1 inoculation. The neurological deficient scores were determined daily.
The water content of brain tissue in the TCS-treated group between 48 hrs and 7 days after HSV-1 inoculation was significantly lower than that in the Model group (P < 0.05), although it was significantly higher than that in the Normal control group (P < 0.05). The viral titer of brain tissue in the TCS-treated group was markedly lower than that in the Model group (1.16 +/- 0.45 vs 2.89 +/- 0.44; P < 0.05) 7 days after HSV-1 inoculation. The neurological deficient scores of the TCS-treated group after 24 hrs of HSV-1 inoculation were significantly lower than that in the Model group but were higher than those of the Normal control group. TCS treatment resulted in alleviated pathological changes of brain tissue compared with the Model group 7 days after HSV-1 inoculation.
TCS has protective effects against infectious brain injury induced by HSV-1 in mice.
天花粉蛋白（TCS）是从传统中药材栝楼的块根中提取的一种核糖体失活蛋白，具有多种药理特性，包括堕胎、抗肿瘤和抗病毒作用。本研究旨在评估TCS对单纯疱疹病毒1型（HSV-1）诱导的小鼠感染性脑损伤的影响。
将90只小鼠随机分为三组：正常对照组（n = 30）、模型组（n = 30）和TCS治疗组（n = 30）。后两组通过颅内接种HSV-1诱导病毒性脑炎。TCS治疗组在接种HSV-1前30分钟注射TCS。在接种HSV-1后1、12、24和48小时以及4和7天测量脑组织含水量。在接种HSV-1后7天检测脑组织病毒滴度和脑组织病理组织学变化。每天测定神经功能缺损评分。
HSV-1接种后48小时至7天，TCS治疗组脑组织含水量显著低于模型组（P < 0.05），尽管显著高于正常对照组（P < 0.05）。HSV-1接种后7天，TCS治疗组脑组织病毒滴度明显低于模型组（1.16 +/- 0.45对2.89 +/- 0.44；P < 0.05）。HSV-1接种24小时后，TCS治疗组的神经功能缺损评分显著低于模型组，但高于正常对照组。与HSV-1接种后7天的模型组相比，TCS治疗减轻了脑组织的病理变化。
TCS对HSV-1诱导的小鼠感染性脑损伤具有保护作用。
