Au Angela, Ha Jinny, Hernandez Mauro, Polotsky Anna, Hungerford David S, Frondoza Carmelita G
Department of Orthopaedic Surgery, Johns Hopkins University, 5601 Loch Raven Blvd., Baltimore, Maryland 21239, USA.
J Biomed Mater Res A. 2006 Dec 1;79(3):512-21. doi: 10.1002/jbm.a.30811.
Metal alloys are used as prosthetic components in the orthopaedic and dental field. However, there is growing concern over the reported leaching of metal ions from implants. Ions released from metals have been thought to be associated with local immune dysfunction, inflammation, and tissue cell death. The objective of our study was to investigate whether nickel(II) and vanadium(V), present at a smaller percentage in most alloys, are cytotoxic to T-lymphocyte cell models. Jurkat T cells possess characteristics similar to human T-lymphocytes and proliferate at a faster rate. Jurkat T cells were incubated with control media alone or with concentrations of 1, 10, and 100 microg/mL of Ni(II) or V(V) for 24 h. Both types of metal ions reduced cell viability and proliferation in a dose-dependent manner. Ni(II) at 10 microg/mL and V(V) at 100 microg/mL activated Caspase-3 expression. Hoechst 33258 staining and transmission electron microscopy revealed chromatin condensation, as well as nuclear blebbing and fragmentation. Induction of DNA fragmentation by Ni(II) at 100 microg/mL was also indicated by agarose electrophoresis. Our observations indicate that Ni and V ions kill T cells via apoptotic and nonapoptotic pathways.
金属合金被用作骨科和牙科领域的假体部件。然而，人们越来越关注报道中植入物金属离子的浸出问题。金属释放的离子被认为与局部免疫功能障碍、炎症和组织细胞死亡有关。我们研究的目的是调查在大多数合金中所占比例较小的镍（II）和钒（V）是否对T淋巴细胞细胞模型具有细胞毒性。Jurkat T细胞具有与人类T淋巴细胞相似的特征，且增殖速度更快。将Jurkat T细胞单独与对照培养基孵育，或与浓度为1、10和100μg/mL的镍（II）或钒（V）孵育24小时。两种类型的金属离子均以剂量依赖的方式降低细胞活力和增殖。10μg/mL的镍（II）和100μg/mL的钒（V）激活了半胱天冬酶-3的表达。Hoechst 33258染色和透射电子显微镜显示染色质浓缩，以及核泡状化和碎片化。琼脂糖电泳也表明100μg/mL的镍（II）诱导了DNA片段化。我们的观察结果表明，镍和钒离子通过凋亡和非凋亡途径杀死T细胞。
