南方菜豆花叶病毒外壳蛋白mRNA的特性及其与基因组RNA的关系。

Characterization of the coat protein mRNA of southern bean mosaic virus and its relationship to the genomic RNA.

作者信息

Ghosh A, Rutgers T, Ke-Qiang M, Kaesberg P

机构信息

Biophysics Laboratory, Graduate School, and Department of Biochemistry, College of Agricultural and Life Sciences, University of Wisconsin-Madison, Madison, Wisconsin 53706.

出版信息

J Virol. 1981 Jul;39(1):87-92. doi: 10.1128/JVI.39.1.87-92.1981.

DOI:10.1128/JVI.39.1.87-92.1981

PMID:16789211

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC171268/

Abstract

RNA isolated from southern bean mosaic virions contains, in small amount, a subgenomic RNA (molecular weight, 0.38 x 10(6)) that serves in vitro as an mRNA for southern bean mosaic virus coat protein. The RNA has a 5'-linked protein indistinguishable from the protein linked to the 5' end of full-length genomic RNA. Its base sequence, determined to 91 bases from the 3' end, is identical to the 3'-terminal sequence of the genomic RNA. The results suggest that the coat protein messenger sequence exists as a "silent" cistron near the 3' end of the genomic RNA.

摘要

从南方菜豆花叶病毒粒子中分离出的RNA含有少量亚基因组RNA（分子量为0.38×10⁶），该亚基因组RNA在体外可作为南方菜豆花叶病毒外壳蛋白的信使核糖核酸。这种RNA具有一个5'-连接蛋白，与连接在全长基因组RNA 5'端的蛋白无法区分。从3'端起测定到91个碱基的碱基序列与基因组RNA的3'-末端序列相同。这些结果表明，外壳蛋白信使序列作为一个“沉默”顺反子存在于基因组RNA的3'端附近。