Ghosh A, Rutgers T, Ke-Qiang M, Kaesberg P
Biophysics Laboratory, Graduate School, and Department of Biochemistry, College of Agricultural and Life Sciences, University of Wisconsin-Madison, Madison, Wisconsin 53706.
J Virol. 1981 Jul;39(1):87-92. doi: 10.1128/JVI.39.1.87-92.1981.
RNA isolated from southern bean mosaic virions contains, in small amount, a subgenomic RNA (molecular weight, 0.38 x 10(6)) that serves in vitro as an mRNA for southern bean mosaic virus coat protein. The RNA has a 5'-linked protein indistinguishable from the protein linked to the 5' end of full-length genomic RNA. Its base sequence, determined to 91 bases from the 3' end, is identical to the 3'-terminal sequence of the genomic RNA. The results suggest that the coat protein messenger sequence exists as a "silent" cistron near the 3' end of the genomic RNA.
从南方菜豆花叶病毒粒子中分离出的RNA含有少量亚基因组RNA(分子量为0.38×10⁶),该亚基因组RNA在体外可作为南方菜豆花叶病毒外壳蛋白的信使核糖核酸。这种RNA具有一个5'-连接蛋白,与连接在全长基因组RNA 5'端的蛋白无法区分。从3'端起测定到91个碱基的碱基序列与基因组RNA的3'-末端序列相同。这些结果表明,外壳蛋白信使序列作为一个“沉默”顺反子存在于基因组RNA的3'端附近。