南方菜豆花叶病毒核糖核酸具有一个5'-连接蛋白，但缺乏3'末端多聚腺苷酸。
Southern bean mosaic viral RNA has a 5'-linked protein but lacks 3' terminal poly(A).
作者信息
Ghosh A, Dasgupta R, Salerno-Rife T, Rutgers T, Kaesberg P
出版信息
Nucleic Acids Res. 1979 Dec 20;7(8):2137-46. doi: 10.1093/nar/7.8.2137.
Abstract
Nuclease digestion of SBMV RNA releases a protein of molecular weight approximately 12,000 in addition to the four mononucleotides. The lack of reactivity of SBMV RNA to polynucleotide kinase and the absence of a capping group suggest that the protein is covalently attached to the 5' end of the RNA. RNA sequencing shows that the 3' terminus of SBMV RNA is not polyadenylated.
摘要
对南方菜豆花叶病毒（SBMV）RNA进行核酸酶消化，除了释放出四种单核苷酸外，还释放出一种分子量约为12,000的蛋白质。SBMV RNA对多核苷酸激酶无反应性且不存在帽状结构，这表明该蛋白质与RNA的5'端共价连接。RNA测序显示，SBMV RNA的3'末端没有聚腺苷酸化。
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