van de Werken Harmen J G, Verhees Corné H, Akerboom Jasper, de Vos Willem M, van der Oost John
Laboratory of Microbiology, Wageningen University, Wageningen, The Netherlands.
FEMS Microbiol Lett. 2006 Jul;260(1):69-76. doi: 10.1111/j.1574-6968.2006.00292.x.
The glycolytic pathway of the hyperthermophilic archaea that belong to the order Thermococcales (Pyrococcus, Thermococcus and Palaeococcus) differs significantly from the canonical Embden-Meyerhof pathway in bacteria and eukarya. This archaeal glycolysis variant consists of several novel enzymes, some of which catalyze unique conversions. Moreover, the enzymes appear not to be regulated allosterically, but rather at transcriptional level. To elucidate details of the gene expression control, the transcription initiation sites of the glycolytic genes in Pyrococcus furiosus have been mapped by primer extension analysis and the obtained promoter sequences have been compared with upstream regions of non-glycolytic genes. Apart from consensus sequences for the general transcription factors (TATA-box and BRE) this analysis revealed the presence of a potential transcription factor binding site (TATCAC-N(5)-GTGATA) in glycolytic and starch utilizing promoters of P. furiosus and several thermococcal species. The absence of this inverted repeat in Pyrococcus abyssi and Pyrococcus horikoshii probably reflects that their reduced catabolic capacity does not require this regulatory system. Moreover, this phyletic pattern revealed a TrmB-like regulator (PF0124 and TK1769) which may be involved in recognizing the repeat. This Thermococcales glycolytic regulon, with more than 20 genes, is the largest regulon that has yet been described for Archaea.
属于热球菌目（嗜热栖热菌属、嗜热栖热球菌属和古球菌属）的嗜热古菌的糖酵解途径与细菌和真核生物中的经典糖酵解途径（Embden-Meyerhof途径）有显著差异。这种古菌糖酵解变体由几种新型酶组成，其中一些酶催化独特的转化反应。此外，这些酶似乎不是通过别构调节，而是在转录水平上进行调节。为了阐明基因表达控制的细节，通过引物延伸分析确定了激烈火球菌中糖酵解基因的转录起始位点，并将获得的启动子序列与非糖酵解基因的上游区域进行了比较。除了一般转录因子的共有序列（TATA盒和BRE）外，该分析还揭示了在激烈火球菌和几种嗜热栖热球菌属物种的糖酵解和淀粉利用启动子中存在一个潜在的转录因子结合位点（TATCAC-N(5)-GTGATA）。深渊嗜热栖热菌和堀越嗜热栖热菌中不存在这种反向重复序列，这可能反映出它们降低的分解代谢能力不需要这种调节系统。此外，这种系统发育模式揭示了一种类似TrmB的调节因子（PF0124和TK1769），它可能参与识别该重复序列。这个包含20多个基因的热球菌目糖酵解调节子是迄今为止已描述的古菌中最大的调节子。
