Bazzichi Laura, Giannaccini Gino, Betti Laura, Mascia Giovanni, Fabbrini Laura, Italiani Paola, De Feo Francesca, Giuliano Tiziana, Giacomelli Camillo, Rossi Alessandra, Lucacchini Antonio, Bombardieri Stefano
Department of Internal Medicine, Division of Rheumatology, University of Pisa, Via Roma 67-56126 Pisa, Italy.
Arthritis Res Ther. 2006;8(4):R99. doi: 10.1186/ar1982.
The aim of the study was to evaluate the kinetic parameters of a specific serotonin transporter (SERT) and serotonin uptake in a mentally healthy subset of patients with fibromyalgia. Platelets were obtained from 40 patients and 38 healthy controls. SERT expression and functionality were evaluated through the measurement of [3H]paroxetine binding and the [3H]serotonin uptake itself. The values of maximal membrane binding capacity (Bmax) were statistically lower in the patients than in the healthy volunteers, whereas the dissociation constant (Kd) did not show any statistically significant variations. Moreover, a decrease in the maximal uptake rate of SERT (Vmax) was demonstrated in the platelets of patients, whereas the Michaelis constant (Km) did not show any statistically significant variations. Symptom severity score (tiredness, tender points index and Fibromyalgia Impact Questionnaire) were negatively correlated with Bmax and with Vmax, and positively correlated with Km. A change in SERT seems to occur in fibromyalgic patients, and it seems to be related to the severity of fibromyalgic symptoms.
该研究的目的是评估纤维肌痛症心理健康亚组患者中特定血清素转运体（SERT）的动力学参数以及血清素摄取情况。从40例患者和38名健康对照者获取血小板。通过测量[3H]帕罗西汀结合以及[3H]血清素摄取本身来评估SERT的表达和功能。患者的最大膜结合容量（Bmax）值在统计学上低于健康志愿者，而解离常数（Kd）未显示任何统计学上的显著差异。此外，患者血小板中SERT的最大摄取速率（Vmax）降低，而米氏常数（Km）未显示任何统计学上的显著差异。症状严重程度评分（疲劳、压痛点指数和纤维肌痛影响问卷）与Bmax和Vmax呈负相关，与Km呈正相关。纤维肌痛症患者似乎发生了SERT的变化，且这似乎与纤维肌痛症状的严重程度有关。
