Gilman S Douglass, Chapman Peter J
Department of Chemistry, Louisiana State University, Baton Rouge, LA, USA.
Methods Mol Biol. 2006;339:187-202. doi: 10.1385/1-59745-076-6:187.
Electrophoretic migration and electroosmotic flow (EOF) combine to determine the migration rate of charged compounds in capillary electrophoresis (CE) and microchip capillary electrophoresis (MCE). Uncontrolled and unmeasured changes in EOF will lead to irreproducible peak migration times and poor peak quantitation. The two most common methods for measuring EOF for CE and MCE are detailed. Experimental results for application of the neutral marker method and the current monitoring method to EC are presented, and related calculations of EOF rates and electroosmotic mobility are described. The strengths and shortcomings of these two EOF measurement techniques are discussed. Additional approaches for studying and measuring EOF and for improving the reproducibility of migration times for CE and MCE are summarized.
在毛细管电泳（CE）和微芯片毛细管电泳（MCE）中，电泳迁移和电渗流（EOF）共同决定带电化合物的迁移速率。EOF的不受控制和未测量的变化将导致峰迁移时间不可重现以及峰定量不佳。详细介绍了用于CE和MCE测量EOF的两种最常用方法。给出了中性标记法和电流监测法应用于CE的实验结果，并描述了EOF速率和电渗迁移率的相关计算。讨论了这两种EOF测量技术的优缺点。总结了研究和测量EOF以及提高CE和MCE迁移时间重现性的其他方法。
