Sundlov Jesse A, Garringer Julie A, Carney Jill M, Reger Albert S, Drake Eric J, Duax William L, Gulick Andrew M
Hauptman-Woodward Medical Research Institute, Buffalo, NY, USA.
Acta Crystallogr D Biol Crystallogr. 2006 Jul;62(Pt 7):734-40. doi: 10.1107/S0907444906015824. Epub 2006 Jun 20.
The Escherichia coli enterobactin synthetic cluster is composed of six proteins, EntA-EntF, that form the enterobactin molecule from three serine molecules and three molecules of 2,3-dihydroxybenzoic acid (DHB). EntC, EntB and EntA catalyze the three-step synthesis of DHB from chorismate. EntA is a member of the short-chain oxidoreductase (SCOR) family of proteins and catalyzes the final step in DHB synthesis, the NAD+-dependent oxidation of 2,3-dihydro-2,3-dihydroxybenzoic acid to DHB. The structure of EntA has been determined by multi-wavelength anomalous dispersion methods. Here, the 2.0 A crystal structure of EntA in the unliganded form is presented. Analysis of the structure in light of recent structural and bioinformatic analysis of other members of the SCOR family provides insight into the residues involved in cofactor and substrate binding.
大肠杆菌肠杆菌素合成簇由六种蛋白质（EntA - EntF）组成，它们从三个丝氨酸分子和三个2,3 - 二羟基苯甲酸（DHB）分子形成肠杆菌素分子。EntC、EntB和EntA催化从分支酸三步合成DHB。EntA是短链氧化还原酶（SCOR）蛋白家族的成员，催化DHB合成的最后一步，即依赖NAD +将2,3 - 二氢 - 2,3 - 二羟基苯甲酸氧化为DHB。EntA的结构已通过多波长反常色散方法确定。这里展示了未结合配体形式的EntA的2.0 Å晶体结构。根据对SCOR家族其他成员最近的结构和生物信息学分析对该结构进行分析，有助于深入了解参与辅因子和底物结合的残基。
